In-cell investigation of the conformational landscape of the GTPase UreG by SDSL-EPR

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2023
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Tamburrini, Ketty Concetta
Leguenno, Hugo
Gerbaud, Guillaume
Etienne, Emilien
Guigliarelli, Bruno
Belle, Valérie
Zambelli, Barbara
Mileo, Elisabetta
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iScience. Elsevier. 2023, 26(10), 107855. eISSN 2589-0042. Available under: doi: 10.1016/j.isci.2023.107855
Zusammenfassung

UreG is a cytosolic GTPase involved in the maturation network of urease, an Ni-containing bacterial enzyme. Previous investigations in vitro showed that UreG features a flexible tertiary organization, making this protein the first enzyme discovered to be intrinsically disordered. To determine whether this heterogeneous behavior is maintained in the protein natural environment, UreG structural dynamics was investigated directly in intact bacteria by in-cell EPR. This approach, based on site-directed spin labeling coupled to electron paramagnetic resonance (SDSL-EPR) spectroscopy, enables the study of proteins in their native environment. The results show that UreG maintains heterogeneous structural landscape in-cell, existing in a conformational ensemble of two major conformers, showing either random coil-like or compact properties. These data support the physiological relevance of the intrinsically disordered nature of UreG and indicates a role of protein flexibility for this specific enzyme, possibly related to the regulation of promiscuous protein interactions for metal ion delivery.

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ISO 690PIERRO, Annalisa, Ketty Concetta TAMBURRINI, Hugo LEGUENNO, Guillaume GERBAUD, Emilien ETIENNE, Bruno GUIGLIARELLI, Valérie BELLE, Barbara ZAMBELLI, Elisabetta MILEO, 2023. In-cell investigation of the conformational landscape of the GTPase UreG by SDSL-EPR. In: iScience. Elsevier. 2023, 26(10), 107855. eISSN 2589-0042. Available under: doi: 10.1016/j.isci.2023.107855
BibTex
@article{Pierro2023Incel-69438,
  year={2023},
  doi={10.1016/j.isci.2023.107855},
  title={In-cell investigation of the conformational landscape of the GTPase UreG by SDSL-EPR},
  number={10},
  volume={26},
  journal={iScience},
  author={Pierro, Annalisa and Tamburrini, Ketty Concetta and Leguenno, Hugo and Gerbaud, Guillaume and Etienne, Emilien and Guigliarelli, Bruno and Belle, Valérie and Zambelli, Barbara and Mileo, Elisabetta},
  note={Article Number: 107855}
}
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