Crystal structures of ternary complexes of archaeal B-family DNA polymerases

Kropp, Heike M.; Betz, Karin; Wirth, Johannes; Diederichs, Kay; Marx, Andreas

Crystal structures of ternary complexes of archaeal B-family DNA polymerases

Dateien

Kropp_2-84drh4h134z39.pdfGröße: 21.08 MBDownloads: 351

Datum

2017

Angaben zur Forschungsförderung

European Union (EU): 339834

Projekt

EvoEPIGEN - Evolved Replication Systems of Epigenetics

Open Access-Veröffentlichung

Open Access Gold

Sammlungen

Chemie
Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

PloS ONE. 2017, 12(12), e0188005. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0188005

Zusammenfassung

Archaeal B-family polymerases drive biotechnology by accepting a wide substrate range of chemically modified nucleotides. By now no structural data for archaeal B-family DNA polymerases in a closed, ternary complex are available, which would be the basis for developing next generation nucleotides. We present the ternary crystal structures of KOD and 9°N DNA polymerases complexed with DNA and the incoming dATP. The structures reveal a third metal ion in the active site, which was so far only observed for the eukaryotic B-family DNA polymerase δ and no other B-family DNA polymerase. The structures reveal a wide inner channel and numerous interactions with the template strand that provide space for modifications within the enzyme and may account for the high processivity, respectively. The crystal structures provide insights into the superiority over other DNA polymerases concerning the acceptance of modified nucleotides.

Fachgebiet (DDC)

540 Chemie

Zitieren

ISO 690

KROPP, Heike M., Karin BETZ, Johannes WIRTH, Kay DIEDERICHS, Andreas MARX, 2017. Crystal structures of ternary complexes of archaeal B-family DNA polymerases. In: PloS ONE. 2017, 12(12), e0188005. eISSN 1932-6203. Available under: doi: 10.1371/journal.pone.0188005

BibTex

@article{Kropp2017Cryst-41015,
  year={2017},
  doi={10.1371/journal.pone.0188005},
  title={Crystal structures of ternary complexes of archaeal B-family DNA polymerases},
  number={12},
  volume={12},
  journal={PloS ONE},
  author={Kropp, Heike M. and Betz, Karin and Wirth, Johannes and Diederichs, Kay and Marx, Andreas},
  note={Article Number: e0188005}
}

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Universitätsbibliographie

Ja