A Need for Speed : Genetic Encoding of Rapid Cycloaddition Chemistries for Protein Labelling in Living Cells

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ChemBioChem. Wiley. 2012, 13(11), pp. 1553-1557. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201200321
Zusammenfassung

Rapid reactions: Several reactants for strain‐promoted cycloaddition reactions (see picture) have been genetically encoded as the side chains of noncanonical amino acids. This results in decisive improvements for the fluorescent labelling of intracellular proteins such as quantitative turnover, completion of labelling reactions within minutes, fluorogenic effects and even partial orthogonality for multicolour labelling.

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ISO 690SCHMIDT, Moritz J., Daniel SUMMERER, 2012. A Need for Speed : Genetic Encoding of Rapid Cycloaddition Chemistries for Protein Labelling in Living Cells. In: ChemBioChem. Wiley. 2012, 13(11), pp. 1553-1557. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201200321
BibTex
@article{Schmidt2012-07-23Speed-48562,
  year={2012},
  doi={10.1002/cbic.201200321},
  title={A Need for Speed : Genetic Encoding of Rapid Cycloaddition Chemistries for Protein Labelling in Living Cells},
  number={11},
  volume={13},
  issn={1439-4227},
  journal={ChemBioChem},
  pages={1553--1557},
  author={Schmidt, Moritz J. and Summerer, Daniel}
}
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