Comamonas testosteroni BR6020 possesses a single genetic locus for extradiol cleavage of protocatechuate

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2001
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Providenti, Miguel A.
Mampel, Jörg
McSween, Scott
Wyndham, R. Campbell
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A key intermediate for biodegradation of various distinct aromatic growth substrates in Comamonas testosteroni is protocatechuate (Pca), which is metabolized by the 4,5-extradiol (meta) ring fission pathway. A locus harbouring genes from C. testosteroni BR6020 was cloned, dubbed pmd, which encodes the enzymes that degrade Pca. The identity of pmdAB, encoding respectively the alpha- and beta-subunit of the Pca ring-cleavage enzyme, was confirmed by N-terminal sequencing and molecular mass determination of both subunits from the separated enzyme. Disruption of pmdA resulted in a strain unable to grow on Pca and a variety of aromatic substrates funnelled through this compound (m- and p-hydroxybenzoate, p-sulfobenzoate, phthalate, isophthalate, terephthalate, vanillate, isovanillate and veratrate). Growth on benzoate and o-aminobenzoate (anthranilate) was not affected in this strain, indicating that these substrates are metabolized via a different lower pathway. Tentative functions for the products of other pmd genes were assigned based on sequence identity and/or similarity to proteins from other proteobacteria involved in uptake or metabolism of aromatic compounds. This study provides evidence for a single lower pathway in C. testosteroni for metabolism of Pca, which is generated by different upper pathways acting on a variety of aromatic substrates.

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570 Biowissenschaften, Biologie
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aromatic, biodegradation, meta ring fission, lig genes
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ISO 690PROVIDENTI, Miguel A., Jörg MAMPEL, Scott MCSWEEN, Alasdair M. COOK, R. Campbell WYNDHAM, 2001. Comamonas testosteroni BR6020 possesses a single genetic locus for extradiol cleavage of protocatechuate. In: Microbiology. 2001, 147(8), pp. 2157-2167. ISSN 1350-0872. eISSN 1465-2080. Available under: doi: 10.1099/00221287-147-8-2157
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@article{Providenti2001Comam-6764,
  year={2001},
  doi={10.1099/00221287-147-8-2157},
  title={Comamonas testosteroni BR6020 possesses a single genetic locus for extradiol cleavage of protocatechuate},
  number={8},
  volume={147},
  issn={1350-0872},
  journal={Microbiology},
  pages={2157--2167},
  author={Providenti, Miguel A. and Mampel, Jörg and McSween, Scott and Cook, Alasdair M. and Wyndham, R. Campbell}
}
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