Degradation of o-xylene and m-xylene by a novel sulfate-reducer belonging to the genus Desulfotomaculum

Lade...
Vorschaubild
Dateien
Datum
2004
Autor:innen
Morasch, Barbara
Tebbe, Christoph C.
Meckenstock, Rainer U.
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
EU-Projektnummer
DFG-Projektnummer
Projekt
Open Access-Veröffentlichung
Sammlungen
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
unikn.publication.listelement.citation.prefix.version.undefined
Archives of Microbiology. 2004, 181(6), pp. 407-417. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s00203-004-0672-6
Zusammenfassung

A strictly anaerobic bacterium, strain OX39, was isolated with o-xylene as organic substrate and sulfate as electron acceptor from an aquifer at a former gasworks plant contaminated with aromatic hydrocarbons. Apart from o-xylene, strain OX39 grew on m-xylene and toluene and all three substrates were oxidized completely to CO2. Induction experiments indicated that o-xylene, m-xylene, and toluene degradation were initiated by different specific enzymes. Methylbenzylsuccinate was identified in supernatants of cultures grown on o-xylene and m-xylene, and benzylsuccinate was detected in supernatants of toluene-grown cells, thus indicating that degradation was initiated in all three cases by fumarate addition to the methyl group. Strain OX39 was sensitive towards sulfide and depended on Fe(II) in the medium as a scavenger of the produced sulfide. Analysis of the PCR-amplified 16S rRNA gene revealed that strain OX39 affiliates with the gram-positive endospore-forming sulfate reducers of the genus Desulfotomaculum and is the first hydrocarbonoxidizing bacterium in this genus.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Sulfate reduction, BTEX, Benzylsuccinate synthase, Desulfotomaculum
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690MORASCH, Barbara, Bernhard SCHINK, Christoph C. TEBBE, Rainer U. MECKENSTOCK, 2004. Degradation of o-xylene and m-xylene by a novel sulfate-reducer belonging to the genus Desulfotomaculum. In: Archives of Microbiology. 2004, 181(6), pp. 407-417. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s00203-004-0672-6
BibTex
@article{Morasch2004Degra-8500,
  year={2004},
  doi={10.1007/s00203-004-0672-6},
  title={Degradation of o-xylene and m-xylene by a novel sulfate-reducer belonging to the genus Desulfotomaculum},
  number={6},
  volume={181},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={407--417},
  author={Morasch, Barbara and Schink, Bernhard and Tebbe, Christoph C. and Meckenstock, Rainer U.}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8500">
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dcterms:bibliographicCitation>First publ. in: Archives of Microbiology 181 (2004), 6, pp. 407-417</dcterms:bibliographicCitation>
    <dc:contributor>Meckenstock, Rainer U.</dc:contributor>
    <dcterms:abstract xml:lang="eng">A strictly anaerobic bacterium, strain OX39, was isolated with o-xylene as organic substrate and sulfate as electron acceptor from an aquifer at a former gasworks plant contaminated with aromatic hydrocarbons. Apart from o-xylene, strain OX39 grew on m-xylene and toluene and all three substrates were oxidized completely to CO2. Induction experiments indicated that o-xylene, m-xylene, and toluene degradation were initiated by different specific enzymes. Methylbenzylsuccinate was identified in supernatants of cultures grown on o-xylene and m-xylene, and benzylsuccinate was detected in supernatants of toluene-grown cells, thus indicating that degradation was initiated in all three cases by fumarate addition to the methyl group. Strain OX39 was sensitive towards sulfide and depended on Fe(II) in the medium as a scavenger of the produced sulfide. Analysis of the PCR-amplified 16S rRNA gene revealed that strain OX39 affiliates with the gram-positive endospore-forming sulfate reducers of the genus Desulfotomaculum and is the first hydrocarbonoxidizing bacterium in this genus.</dcterms:abstract>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8500/1/Degradation_of_o_xylene_and_m_xylene_by_a_2004.pdf"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8500/1/Degradation_of_o_xylene_and_m_xylene_by_a_2004.pdf"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:44:11Z</dc:date>
    <dc:creator>Tebbe, Christoph C.</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:44:11Z</dcterms:available>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:format>application/pdf</dc:format>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8500"/>
    <dc:contributor>Tebbe, Christoph C.</dc:contributor>
    <dc:contributor>Morasch, Barbara</dc:contributor>
    <dc:creator>Morasch, Barbara</dc:creator>
    <dcterms:issued>2004</dcterms:issued>
    <dc:creator>Schink, Bernhard</dc:creator>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dcterms:title>Degradation of o-xylene and m-xylene by a novel sulfate-reducer belonging to the genus Desulfotomaculum</dcterms:title>
    <dc:creator>Meckenstock, Rainer U.</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Schink, Bernhard</dc:contributor>
    <dc:language>eng</dc:language>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet