Degradation of o-xylene and m-xylene by a novel sulfate-reducer belonging to the genus Desulfotomaculum

Loading...
Thumbnail Image
Date
2004
Authors
Morasch, Barbara
Tebbe, Christoph C.
Meckenstock, Rainer U.
Editors
Contact
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
DOI (citable link)
ArXiv-ID
International patent number
EU project number
Project
Open Access publication
Collections
Restricted until
Title in another language
Research Projects
Organizational Units
Journal Issue
Publication type
Journal article
Publication status
Published in
Archives of Microbiology ; 181 (2004), 6. - pp. 407-417. - ISSN 0302-8933. - eISSN 1432-072X
Abstract
A strictly anaerobic bacterium, strain OX39, was isolated with o-xylene as organic substrate and sulfate as electron acceptor from an aquifer at a former gasworks plant contaminated with aromatic hydrocarbons. Apart from o-xylene, strain OX39 grew on m-xylene and toluene and all three substrates were oxidized completely to CO2. Induction experiments indicated that o-xylene, m-xylene, and toluene degradation were initiated by different specific enzymes. Methylbenzylsuccinate was identified in supernatants of cultures grown on o-xylene and m-xylene, and benzylsuccinate was detected in supernatants of toluene-grown cells, thus indicating that degradation was initiated in all three cases by fumarate addition to the methyl group. Strain OX39 was sensitive towards sulfide and depended on Fe(II) in the medium as a scavenger of the produced sulfide. Analysis of the PCR-amplified 16S rRNA gene revealed that strain OX39 affiliates with the gram-positive endospore-forming sulfate reducers of the genus Desulfotomaculum and is the first hydrocarbonoxidizing bacterium in this genus.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
Sulfate reduction,BTEX,Benzylsuccinate synthase,Desulfotomaculum
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690MORASCH, Barbara, Bernhard SCHINK, Christoph C. TEBBE, Rainer U. MECKENSTOCK, 2004. Degradation of o-xylene and m-xylene by a novel sulfate-reducer belonging to the genus Desulfotomaculum. In: Archives of Microbiology. 181(6), pp. 407-417. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s00203-004-0672-6
BibTex
@article{Morasch2004Degra-8500,
  year={2004},
  doi={10.1007/s00203-004-0672-6},
  title={Degradation of o-xylene and m-xylene by a novel sulfate-reducer belonging to the genus Desulfotomaculum},
  number={6},
  volume={181},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={407--417},
  author={Morasch, Barbara and Schink, Bernhard and Tebbe, Christoph C. and Meckenstock, Rainer U.}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8500">
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dcterms:bibliographicCitation>First publ. in: Archives of Microbiology 181 (2004), 6, pp. 407-417</dcterms:bibliographicCitation>
    <dc:contributor>Meckenstock, Rainer U.</dc:contributor>
    <dcterms:abstract xml:lang="eng">A strictly anaerobic bacterium, strain OX39, was isolated with o-xylene as organic substrate and sulfate as electron acceptor from an aquifer at a former gasworks plant contaminated with aromatic hydrocarbons. Apart from o-xylene, strain OX39 grew on m-xylene and toluene and all three substrates were oxidized completely to CO2. Induction experiments indicated that o-xylene, m-xylene, and toluene degradation were initiated by different specific enzymes. Methylbenzylsuccinate was identified in supernatants of cultures grown on o-xylene and m-xylene, and benzylsuccinate was detected in supernatants of toluene-grown cells, thus indicating that degradation was initiated in all three cases by fumarate addition to the methyl group. Strain OX39 was sensitive towards sulfide and depended on Fe(II) in the medium as a scavenger of the produced sulfide. Analysis of the PCR-amplified 16S rRNA gene revealed that strain OX39 affiliates with the gram-positive endospore-forming sulfate reducers of the genus Desulfotomaculum and is the first hydrocarbonoxidizing bacterium in this genus.</dcterms:abstract>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8500/1/Degradation_of_o_xylene_and_m_xylene_by_a_2004.pdf"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8500/1/Degradation_of_o_xylene_and_m_xylene_by_a_2004.pdf"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:44:11Z</dc:date>
    <dc:creator>Tebbe, Christoph C.</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:44:11Z</dcterms:available>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:format>application/pdf</dc:format>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8500"/>
    <dc:contributor>Tebbe, Christoph C.</dc:contributor>
    <dc:contributor>Morasch, Barbara</dc:contributor>
    <dc:creator>Morasch, Barbara</dc:creator>
    <dcterms:issued>2004</dcterms:issued>
    <dc:creator>Schink, Bernhard</dc:creator>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dcterms:title>Degradation of o-xylene and m-xylene by a novel sulfate-reducer belonging to the genus Desulfotomaculum</dcterms:title>
    <dc:creator>Meckenstock, Rainer U.</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Schink, Bernhard</dc:contributor>
    <dc:language>eng</dc:language>
  </rdf:Description>
</rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Contact
URL of original publication
Test date of URL
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
Yes
Refereed