Degradation of o-xylene and m-xylene by a novel sulfate-reducer belonging to the genus Desulfotomaculum

Morasch, Barbara; Schink, Bernhard; Tebbe, Christoph C.; Meckenstock, Rainer U.

Degradation of o-xylene and m-xylene by a novel sulfate-reducer belonging to the genus Desulfotomaculum

Dateien

Degradation_of_o_xylene_and_m_xylene_by_a_2004.pdfGröße: 342.21 KBDownloads: 670

Datum

2004

Autor:innen

Morasch, Barbara

Schink, Bernhard

Tebbe, Christoph C.

Meckenstock, Rainer U.

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Archives of Microbiology. 2004, 181(6), pp. 407-417. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s00203-004-0672-6

Zusammenfassung

A strictly anaerobic bacterium, strain OX39, was isolated with o-xylene as organic substrate and sulfate as electron acceptor from an aquifer at a former gasworks plant contaminated with aromatic hydrocarbons. Apart from o-xylene, strain OX39 grew on m-xylene and toluene and all three substrates were oxidized completely to CO2. Induction experiments indicated that o-xylene, m-xylene, and toluene degradation were initiated by different specific enzymes. Methylbenzylsuccinate was identified in supernatants of cultures grown on o-xylene and m-xylene, and benzylsuccinate was detected in supernatants of toluene-grown cells, thus indicating that degradation was initiated in all three cases by fumarate addition to the methyl group. Strain OX39 was sensitive towards sulfide and depended on Fe(II) in the medium as a scavenger of the produced sulfide. Analysis of the PCR-amplified 16S rRNA gene revealed that strain OX39 affiliates with the gram-positive endospore-forming sulfate reducers of the genus Desulfotomaculum and is the first hydrocarbonoxidizing bacterium in this genus.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Sulfate reduction, BTEX, Benzylsuccinate synthase, Desulfotomaculum

Zitieren

ISO 690

MORASCH, Barbara, Bernhard SCHINK, Christoph C. TEBBE, Rainer U. MECKENSTOCK, 2004. Degradation of o-xylene and m-xylene by a novel sulfate-reducer belonging to the genus Desulfotomaculum. In: Archives of Microbiology. 2004, 181(6), pp. 407-417. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s00203-004-0672-6

BibTex

@article{Morasch2004Degra-8500,
  year={2004},
  doi={10.1007/s00203-004-0672-6},
  title={Degradation of o-xylene and m-xylene by a novel sulfate-reducer belonging to the genus Desulfotomaculum},
  number={6},
  volume={181},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={407--417},
  author={Morasch, Barbara and Schink, Bernhard and Tebbe, Christoph C. and Meckenstock, Rainer U.}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8500">
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dcterms:bibliographicCitation>First publ. in: Archives of Microbiology 181 (2004), 6, pp. 407-417</dcterms:bibliographicCitation>
    <dc:contributor>Meckenstock, Rainer U.</dc:contributor>
    <dcterms:abstract xml:lang="eng">A strictly anaerobic bacterium, strain OX39, was isolated with o-xylene as organic substrate and sulfate as electron acceptor from an aquifer at a former gasworks plant contaminated with aromatic hydrocarbons. Apart from o-xylene, strain OX39 grew on m-xylene and toluene and all three substrates were oxidized completely to CO2. Induction experiments indicated that o-xylene, m-xylene, and toluene degradation were initiated by different specific enzymes. Methylbenzylsuccinate was identified in supernatants of cultures grown on o-xylene and m-xylene, and benzylsuccinate was detected in supernatants of toluene-grown cells, thus indicating that degradation was initiated in all three cases by fumarate addition to the methyl group. Strain OX39 was sensitive towards sulfide and depended on Fe(II) in the medium as a scavenger of the produced sulfide. Analysis of the PCR-amplified 16S rRNA gene revealed that strain OX39 affiliates with the gram-positive endospore-forming sulfate reducers of the genus Desulfotomaculum and is the first hydrocarbonoxidizing bacterium in this genus.</dcterms:abstract>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8500/1/Degradation_of_o_xylene_and_m_xylene_by_a_2004.pdf"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8500/1/Degradation_of_o_xylene_and_m_xylene_by_a_2004.pdf"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:44:11Z</dc:date>
    <dc:creator>Tebbe, Christoph C.</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:44:11Z</dcterms:available>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:format>application/pdf</dc:format>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8500"/>
    <dc:contributor>Tebbe, Christoph C.</dc:contributor>
    <dc:contributor>Morasch, Barbara</dc:contributor>
    <dc:creator>Morasch, Barbara</dc:creator>
    <dcterms:issued>2004</dcterms:issued>
    <dc:creator>Schink, Bernhard</dc:creator>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dcterms:title>Degradation of o-xylene and m-xylene by a novel sulfate-reducer belonging to the genus Desulfotomaculum</dcterms:title>
    <dc:creator>Meckenstock, Rainer U.</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Schink, Bernhard</dc:contributor>
    <dc:language>eng</dc:language>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja