Publikation:

Curvature Elasticity and Refolding of OmpA in Large Unilamellar Vesicles

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2006

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Pocanschi, Cosmin L.
Marsh, Derek

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Biophysical Journal : Biophysical Letters. 2006, 91(8), pp. L75-L77. ISSN 0006-3495. Available under: doi: 10.1529/biophysj.106.091439

Zusammenfassung

The stability of OmpA in large unilamellar vesicles of dilauroyl phosphatidylcholine was studied using different concentrations of urea. The effective energy of unfolding, as determined from refolding experiments, is greater than that for small sonicated unilamellar vesicles by an amount that is compatible with estimates of the elastic energy of highly curved vesicles. The on-rate for refolding and insertion is slower for large unilamellar vesicles than for small unilamellar vesicles, which indicates a contribution of vesicle strain also to the free energy of the transition state.

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570 Biowissenschaften, Biologie

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ISO 690POCANSCHI, Cosmin L., Geetika J. PATEL, Derek MARSH, Jörg KLEINSCHMIDT, 2006. Curvature Elasticity and Refolding of OmpA in Large Unilamellar Vesicles. In: Biophysical Journal : Biophysical Letters. 2006, 91(8), pp. L75-L77. ISSN 0006-3495. Available under: doi: 10.1529/biophysj.106.091439
BibTex
@article{Pocanschi2006Curva-8331,
  year={2006},
  doi={10.1529/biophysj.106.091439},
  title={Curvature Elasticity and Refolding of OmpA in Large Unilamellar Vesicles},
  number={8},
  volume={91},
  issn={0006-3495},
  journal={Biophysical Journal : Biophysical Letters},
  pages={L75--L77},
  author={Pocanschi, Cosmin L. and Patel, Geetika J. and Marsh, Derek and Kleinschmidt, Jörg}
}
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