Attenuated amyloid-β aggregation and neurotoxicity owing to methionine oxidation

Lade...
Vorschaubild
Dateien
Johansson_2-7gt4fpo0ho0b5.pdf
Johansson_2-7gt4fpo0ho0b5.pdfGröße: 194.52 KBDownloads: 448
Datum
2007
Autor:innen
Johansson, Ann-Sofi
Bergquist, Jonas
Volbracht, Christiane
Päiviö, Anna
Lannfelt, Lars
Westlind-Danielsson, Anita
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Neuroreport. 2007, 18(6), pp. 559-563. ISSN 0959-4965. eISSN 1473-558X. Available under: doi: 10.1097/WNR.0b013e3280b07c21
Zusammenfassung

Aggregation of the amyloid-β (Aβ) peptide into amyloid plaques is a characteristic feature of Alzheimer's disease neuropathogenesis. We and others have previously demonstrated delayed Aβ aggregation as a consequence of oxidizing a single methionine residue at position 35 (Met-35). Here, we examined the consequences of Met-35 oxidation on the extremely aggregation-prone peptides Aβ1-42 and Aβ1-40Arctic with respect to protofibril and oligomer formation as well as neurotoxicity. Size exclusion chromatography and mass spectrometry demonstrated that monomer/dimers prevailed over larger oligomers after oxidizing Met-35, and consequently protofibril formation and aggregation of both A[beta]1-42 and Aβ1-40Arctic were delayed. The oxidized peptides completely lacked neurotoxic effects in cortical neuronal cultures under these conditions, in contrast to the neurotoxic properties of the unoxidized peptides. We conclude that oxidation of Met-35 significantly attenuates aggregation of Aβ1-42 and Aβ1-40Arctic, and thereby reduces neurotoxicity.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
amyloid-β, methionine 35, neurotoxicity, oligomers, oxidation, protofibrils
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690JOHANSSON, Ann-Sofi, Jonas BERGQUIST, Christiane VOLBRACHT, Anna PÄIVIÖ, Marcel LEIST, Lars LANNFELT, Anita WESTLIND-DANIELSSON, 2007. Attenuated amyloid-β aggregation and neurotoxicity owing to methionine oxidation. In: Neuroreport. 2007, 18(6), pp. 559-563. ISSN 0959-4965. eISSN 1473-558X. Available under: doi: 10.1097/WNR.0b013e3280b07c21
BibTex
@article{Johansson2007-04-16Atten-40765,
  year={2007},
  doi={10.1097/WNR.0b013e3280b07c21},
  title={Attenuated amyloid-β aggregation and neurotoxicity owing to methionine oxidation},
  number={6},
  volume={18},
  issn={0959-4965},
  journal={Neuroreport},
  pages={559--563},
  author={Johansson, Ann-Sofi and Bergquist, Jonas and Volbracht, Christiane and Päiviö, Anna and Leist, Marcel and Lannfelt, Lars and Westlind-Danielsson, Anita}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/40765">
    <dcterms:abstract xml:lang="eng">Aggregation of the amyloid-β (Aβ) peptide into amyloid plaques is a characteristic feature of Alzheimer's disease neuropathogenesis. We and others have previously demonstrated delayed Aβ aggregation as a consequence of oxidizing a single methionine residue at position 35 (Met-35). Here, we examined the consequences of Met-35 oxidation on the extremely aggregation-prone peptides Aβ1-42 and Aβ1-40Arctic with respect to protofibril and oligomer formation as well as neurotoxicity. Size exclusion chromatography and mass spectrometry demonstrated that monomer/dimers prevailed over larger oligomers after oxidizing Met-35, and consequently protofibril formation and aggregation of both A[beta]1-42 and Aβ1-40Arctic were delayed. The oxidized peptides completely lacked neurotoxic effects in cortical neuronal cultures under these conditions, in contrast to the neurotoxic properties of the unoxidized peptides. We conclude that oxidation of Met-35 significantly attenuates aggregation of Aβ1-42 and Aβ1-40Arctic, and thereby reduces neurotoxicity.</dcterms:abstract>
    <dc:contributor>Johansson, Ann-Sofi</dc:contributor>
    <dcterms:title>Attenuated amyloid-β aggregation and neurotoxicity owing to methionine oxidation</dcterms:title>
    <dc:creator>Volbracht, Christiane</dc:creator>
    <dc:creator>Leist, Marcel</dc:creator>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:creator>Päiviö, Anna</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/40765/1/Johansson_2-7gt4fpo0ho0b5.pdf"/>
    <dc:contributor>Päiviö, Anna</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <dc:creator>Lannfelt, Lars</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Johansson, Ann-Sofi</dc:creator>
    <dc:creator>Westlind-Danielsson, Anita</dc:creator>
    <dc:contributor>Leist, Marcel</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-11-28T15:32:19Z</dc:date>
    <dc:contributor>Lannfelt, Lars</dc:contributor>
    <dc:contributor>Volbracht, Christiane</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-11-28T15:32:19Z</dcterms:available>
    <dc:language>eng</dc:language>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/40765/1/Johansson_2-7gt4fpo0ho0b5.pdf"/>
    <dc:creator>Bergquist, Jonas</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Bergquist, Jonas</dc:contributor>
    <dcterms:issued>2007-04-16</dcterms:issued>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/40765"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Westlind-Danielsson, Anita</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen