Hydroquinone degradation via reductive dehydroxylation of gentisyl-CoA by a strictly anaerobic fermenting bacterium

Gorny, Norbert; Schink, Bernhard

Hydroquinone degradation via reductive dehydroxylation of gentisyl-CoA by a strictly anaerobic fermenting bacterium

Files

Hydroquinone_degradation_via_reductive_1994.pdf(752.16 KB)

Date

1994

Authors

Gorny, Norbert

Schink, Bernhard

Publication type

Journal article

Published in

Archives of Microbiology ; 161 (1994), 1. - pp. 25-32. - ISSN 0302-8933. - eISSN 1432-072X

Abstract

Anaerobic degradation of hydroquinone was studied with the fermenting bacterium strain HQG61. The rate of hydroquinone degradation by dense cell suspensions was dramatically accelerated by addition of NaHCO3. During fermentation of hydroquinone in the presence of 14C-Na2CO3 benzoate was formed as a labelled product, indicating an initial ortho-carboxylation of hydroquinone to gentisate. Gentisate was activated to the corresponding CoA-ester in a CoA ligase reaction at a specific activity of 0.15 gmol x rain- 1 x mg protein- 1. Gentisyl-CoA was reduced to benzoyl-CoA with reduced methyl viologen as electron donor by simultaneous reductive elimination of both the ortho and meta hydroxyl group. The specific activity of this novel gentisyl-CoA reductase was 17 nmol x rain- 1 x mg protein- 1. Further degradation to acetate was catalyzed by enzymes which occur also in other bacteria degrading aromatic compounds via benzoyl-CoA.

Subject (DDC)

570 Biosciences, Biology

Keywords

Anaerobic degradation,Aromatic compounds,Gentisate,Carboxylation,Reductive elimination,Benzoyl-CoA pathway

Cite This

ISO 690

GORNY, Norbert, Bernhard SCHINK, 1994. Hydroquinone degradation via reductive dehydroxylation of gentisyl-CoA by a strictly anaerobic fermenting bacterium. In: Archives of Microbiology. 161(1), pp. 25-32. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00248890

BibTex

@article{Gorny1994Hydro-6782,
  year={1994},
  doi={10.1007/BF00248890},
  title={Hydroquinone degradation via reductive dehydroxylation of gentisyl-CoA by a strictly anaerobic fermenting bacterium},
  number={1},
  volume={161},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={25--32},
  author={Gorny, Norbert and Schink, Bernhard}
}

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