Calpain inhibitors prevent nitric oxidetriggered excitotoxic apoptosis

Volbracht, Christiane; Fava, Eugenio; Leist, Marcel; Nicotera, Pierluigi

Calpain inhibitors prevent nitric oxidetriggered excitotoxic apoptosis

Files

cv_calpain_2001.pdf(463.96 KB)

Date

2001

Authors

Volbracht, Christiane

Fava, Eugenio

Leist, Marcel

Nicotera, Pierluigi

Publication type

Journal article

Published in

NeuroReport ; 12 (2001), 17. - pp. 3645-3648

Abstract

The pathogenesis of some neurodegenerative disorders has been linked to excitotoxicity, excess generation of nitric oxide (NO) and apoptosis. Here, we used a model of NO-triggered neuronal apoptosis that was strictly dependent on autocrine NMDA receptor (NMDA-R) activation and intracellular Ca2 increase. We investigated the efficiency and potentially beneficial effects of calpain inhibition. Three calpain inhibitors that prevented intracellular fodrin proteolysis also blocked apoptotic features such as decrease in mitochondrial membrane potential, chromatin breakdown, and subsequent death of cerebellar granule neurons exposed to NO donors (S-nitroso- L-glutathione, S-nitroso-N-acetyl D,L-penicillamine, and diethylamino- diazenolate-2-oxide). Since inhibitors did not interfere with NMDA-R activation, we suggest that block of calpains blunts NO-triggered neuronal apoptosis by stopping the cascade downstream of primary autocrine excitotoxic events.

Subject (DDC)

570 Biosciences, Biology

Keywords

Apoptosis,Calpains,Excitotoxicity,Mitochondria,Nitric oxide

Cite This

ISO 690

VOLBRACHT, Christiane, Eugenio FAVA, Marcel LEIST, Pierluigi NICOTERA, 2001. Calpain inhibitors prevent nitric oxidetriggered excitotoxic apoptosis. In: NeuroReport. 12(17), pp. 3645-3648

BibTex

@article{Volbracht2001Calpa-7742,
  year={2001},
  title={Calpain inhibitors prevent nitric oxidetriggered excitotoxic apoptosis},
  number={17},
  volume={12},
  journal={NeuroReport},
  pages={3645--3648},
  author={Volbracht, Christiane and Fava, Eugenio and Leist, Marcel and Nicotera, Pierluigi}
}

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Bibliography of Konstanz

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