Growth temperature-dependent expression of structural variants of Listeria monocytogenes lipoteichoic acid
Growth temperature-dependent expression of structural variants of Listeria monocytogenes lipoteichoic acid
No Thumbnail Available
Files
There are no files associated with this item.
Date
2011
Authors
Dehus, Oliver
Pfitzenmaier, Markus
Stuebs, Gunthard
Fischer, Natalie
Schwaeble, Wilhelm
Morath, Siegfried
Geyer, Armin
Hermann, Corinna
Editors
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
DOI (citable link)
International patent number
Link to the license
oops
EU project number
Project
Open Access publication
Collections
Title in another language
Publication type
Journal article
Publication status
Published
Published in
Immunobiology ; 216 (2011), 1-2. - pp. 24-31. - Elsevier. - ISSN 0171-2985. - eISSN 1878-3279
Abstract
Investigating the expression of lipoteichoic acid (LTA) from Listeria monocytogenes, we found two distinct structural variants of LTA (LTA1 and LTA2) using NMR and MS technology. While both LTA consisted of a poly-glycerophosphate backbone (differing in length) bound via a disaccharide to a diacyl-glycerol moiety, one LTA type (LTA2) possessed a second diacyl-glycerol moiety linked to the disaccharide via a phosphodiester. As examined in vitro, LTA2 in contrast to LTA1 failed to activate the l-ficolin dependent pathway of complement. Most interestingly, growth temperature had a strong influence on the expression levels of LTA1 and LTA2 in the cell wall: while the amount of LTA1 was comparable, the expression of LTA2 was low when Listeria had grown at room temperature (ratio of LTA1 to LTA2 was 1:0.06), but increased when Listeria had been cultivated at 37 °C (ratio of LTA1 to LTA2 was 1:0.68). The observed shift in LTA expression, probably accompanying the switch from the saprophytic to the virulent entity, indicates an important adaptation to the different structural requirements inside the host cells.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
Complement; Lipoteichoic acid; Listeria monocytogenes; Structural analysis
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690
DEHUS, Oliver, Markus PFITZENMAIER, Gunthard STUEBS, Natalie FISCHER, Wilhelm SCHWAEBLE, Siegfried MORATH, Thomas HARTUNG, Armin GEYER, Corinna HERMANN, 2011. Growth temperature-dependent expression of structural variants of Listeria monocytogenes lipoteichoic acid. In: Immunobiology. Elsevier. 216(1-2), pp. 24-31. ISSN 0171-2985. eISSN 1878-3279. Available under: doi: 10.1016/j.imbio.2010.03.008BibTex
@article{Dehus2011Growt-50400, year={2011}, doi={10.1016/j.imbio.2010.03.008}, title={Growth temperature-dependent expression of structural variants of Listeria monocytogenes lipoteichoic acid}, number={1-2}, volume={216}, issn={0171-2985}, journal={Immunobiology}, pages={24--31}, author={Dehus, Oliver and Pfitzenmaier, Markus and Stuebs, Gunthard and Fischer, Natalie and Schwaeble, Wilhelm and Morath, Siegfried and Hartung, Thomas and Geyer, Armin and Hermann, Corinna} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/50400"> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Pfitzenmaier, Markus</dc:contributor> <dc:creator>Geyer, Armin</dc:creator> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-07-30T09:52:03Z</dc:date> <dc:creator>Morath, Siegfried</dc:creator> <dc:contributor>Fischer, Natalie</dc:contributor> <dcterms:abstract xml:lang="eng">Investigating the expression of lipoteichoic acid (LTA) from Listeria monocytogenes, we found two distinct structural variants of LTA (LTA1 and LTA2) using NMR and MS technology. While both LTA consisted of a poly-glycerophosphate backbone (differing in length) bound via a disaccharide to a diacyl-glycerol moiety, one LTA type (LTA2) possessed a second diacyl-glycerol moiety linked to the disaccharide via a phosphodiester. As examined in vitro, LTA2 in contrast to LTA1 failed to activate the l-ficolin dependent pathway of complement. Most interestingly, growth temperature had a strong influence on the expression levels of LTA1 and LTA2 in the cell wall: while the amount of LTA1 was comparable, the expression of LTA2 was low when Listeria had grown at room temperature (ratio of LTA1 to LTA2 was 1:0.06), but increased when Listeria had been cultivated at 37 °C (ratio of LTA1 to LTA2 was 1:0.68). The observed shift in LTA expression, probably accompanying the switch from the saprophytic to the virulent entity, indicates an important adaptation to the different structural requirements inside the host cells.</dcterms:abstract> <dc:contributor>Stuebs, Gunthard</dc:contributor> <dc:creator>Dehus, Oliver</dc:creator> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:contributor>Geyer, Armin</dc:contributor> <dc:language>eng</dc:language> <dc:creator>Hermann, Corinna</dc:creator> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dc:contributor>Schwaeble, Wilhelm</dc:contributor> <dc:creator>Hartung, Thomas</dc:creator> <dcterms:issued>2011</dcterms:issued> <dcterms:title>Growth temperature-dependent expression of structural variants of Listeria monocytogenes lipoteichoic acid</dcterms:title> <dc:contributor>Dehus, Oliver</dc:contributor> <dc:creator>Fischer, Natalie</dc:creator> <dc:contributor>Hartung, Thomas</dc:contributor> <dc:creator>Schwaeble, Wilhelm</dc:creator> <dc:creator>Pfitzenmaier, Markus</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/> <dc:contributor>Morath, Siegfried</dc:contributor> <dc:creator>Stuebs, Gunthard</dc:creator> <dc:contributor>Hermann, Corinna</dc:contributor> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-07-30T09:52:03Z</dcterms:available> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/50400"/> </rdf:Description> </rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
Yes
Refereed
Yes