Investigation of active crystal morphogenesis peptide sequences from peptide libraries by crystallization on peptide functionalized beads

Lade...
Vorschaubild
Dateien
12790.pdf
12790.pdfGröße: 2.45 MBDownloads: 959
Datum
2010
Autor:innen
Krattiger, Philipp
Nassif, Nadine
Mastai, Yitzhak
Wennemers, Helma
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Zusammenfassung

In this study, split-and-mix peptide libraries from one to four amino acids bound to functionalized beads were used to identify active morphogenesis peptides for the systems CaCO3 and dl-alanine. Density gradient ultracentrifugation was used to remove all beads without crystals as well as crystals homogeneously nucleated in solution. From the remaining fractions, beads were selected, which account for differences in crystal morphologies from the default crystal morphology on a micrometer scale and their amino acid sequence was analyzed. Our results show that multiple and different peptide sequences are found to be active in the morphogenesis of CaCO3 and dl-alanine. It was not possible to find a correlation, which connects active single amino acids with the sequences of di-, tri- and tetrapeptides. However, peptide charge was found to be important for morphogenesis. Peptides active in CaCO3 morphogenesis were enriched in basic amino acids while those active for dl-alanine morphogenesis contained more acidic amino acids. This can be explained by charge charge interactions of the crystallizing species with the countercharged peptide moieties. Tests for chiral separation for the dl-alanine system showed that with the applied oligopeptide libraries, no enantioselective crystallization was achieved to a significant extent. The presented combinatorial crystallization assay provides an easy tool for crystallization control, which can be used for the straightforward selection of interesting species under a light microscope. However, suitable staining techniques to identify individual beads with crystals, which are for example a pure enantiomer still need to be developed.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
Schlagwörter
Split-and-mix peptide libraries, CaCO3, dl-Alanine, Morphogenesis, Crystallization control
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690KRATTIGER, Philipp, Nadine NASSIF, Antje VÖLKEL, Yitzhak MASTAI, Helma WENNEMERS, Helmut CÖLFEN, 2010. Investigation of active crystal morphogenesis peptide sequences from peptide libraries by crystallization on peptide functionalized beads. In: Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2010, 354(1-3), pp. 218-225. ISSN 0927-7757. Available under: doi: 10.1016/j.colsurfa.2009.09.031
BibTex
@article{Krattiger2010Inves-10003,
  year={2010},
  doi={10.1016/j.colsurfa.2009.09.031},
  title={Investigation of active crystal morphogenesis peptide sequences from peptide libraries by crystallization on peptide functionalized beads},
  number={1-3},
  volume={354},
  issn={0927-7757},
  journal={Colloids and Surfaces A: Physicochemical and Engineering Aspects},
  pages={218--225},
  author={Krattiger, Philipp and Nassif, Nadine and Völkel, Antje and Mastai, Yitzhak and Wennemers, Helma and Cölfen, Helmut}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/10003">
    <dc:creator>Völkel, Antje</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/10003"/>
    <dcterms:title>Investigation of active crystal morphogenesis peptide sequences from peptide libraries by crystallization on peptide functionalized beads</dcterms:title>
    <dcterms:abstract xml:lang="eng">In this study, split-and-mix peptide libraries from one to four amino acids bound to functionalized beads were used to identify active morphogenesis peptides for the systems CaCO3 and dl-alanine. Density gradient ultracentrifugation was used to remove all beads without crystals as well as crystals homogeneously nucleated in solution. From the remaining fractions, beads were selected, which account for differences in crystal morphologies from the default crystal morphology on a micrometer scale and their amino acid sequence was analyzed. Our results show that multiple and different peptide sequences are found to be active in the morphogenesis of CaCO3 and dl-alanine. It was not possible to find a correlation, which connects active single amino acids with the sequences of di-, tri- and tetrapeptides. However, peptide charge was found to be important for morphogenesis. Peptides active in CaCO3 morphogenesis were enriched in basic amino acids while those active for dl-alanine morphogenesis contained more acidic amino acids. This can be explained by charge charge interactions of the crystallizing species with the countercharged peptide moieties. Tests for chiral separation for the dl-alanine system showed that with the applied oligopeptide libraries, no enantioselective crystallization was achieved to a significant extent. The presented combinatorial crystallization assay provides an easy tool for crystallization control, which can be used for the straightforward selection of interesting species under a light microscope. However, suitable staining techniques to identify individual beads with crystals, which are for example a pure enantiomer still need to be developed.</dcterms:abstract>
    <dc:contributor>Krattiger, Philipp</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:creator>Wennemers, Helma</dc:creator>
    <dc:language>eng</dc:language>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:15:54Z</dcterms:available>
    <dc:contributor>Wennemers, Helma</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/10003/1/12790.pdf"/>
    <dc:creator>Nassif, Nadine</dc:creator>
    <dc:creator>Cölfen, Helmut</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:format>application/pdf</dc:format>
    <dc:creator>Mastai, Yitzhak</dc:creator>
    <dc:creator>Krattiger, Philipp</dc:creator>
    <dc:contributor>Völkel, Antje</dc:contributor>
    <dc:contributor>Cölfen, Helmut</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Mastai, Yitzhak</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:15:54Z</dc:date>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/10003/1/12790.pdf"/>
    <dcterms:bibliographicCitation>First publ. in: Colloids and Surfaces A: Physicochemical and Engineering Aspects 354 (2010), 1-3, pp. 218-225</dcterms:bibliographicCitation>
    <dc:contributor>Nassif, Nadine</dc:contributor>
    <dcterms:issued>2010</dcterms:issued>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen