Publikation: Protein Spin Labeling with a Photocaged Nitroxide Using Diels-Alder Chemistry
Dateien
Datum
Herausgeber:innen
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
URI (zitierfähiger Link)
DOI (zitierfähiger Link)
Internationale Patentnummer
Link zur Lizenz
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Sammlungen
Core Facility der Universität Konstanz
Titel in einer weiteren Sprache
Publikationstyp
Publikationsstatus
Erschienen in
Zusammenfassung
EPR spectroscopy of diamagnetic bio-macromolecules is based on site-directed spin labeling (SDSL). Here, we present a novel labeling strategy for proteins. We developed and synthesized a nitroxide-based spin label that can be ligated to proteins by an inverse-electron-demand Diels-Alder (DAinv) cycloaddition to genetically encoded non-canonical amino acids (ncAA). The nitroxide moiety is shielded by a photoremovable protecting group (PPG) with an attached tetraethylene glycol unit to achieve water solubility. We demonstrate SDSL of two model proteins with the PaNDA (Photoactivatable Nitroxide for DAinv reaction) label. Our strategy features high reaction rates combined with high selectivity, and the possibility to deprotect the nitroxide in E. coli lysate.
Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
Schlagwörter
Konferenz
Rezension
Zitieren
ISO 690
KUGELE, Anandi, Bjarne SILKENATH, Jakob LANGER, Valentin WITTMANN, Malte DRESCHER, 2019. Protein Spin Labeling with a Photocaged Nitroxide Using Diels-Alder Chemistry. In: Chembiochem. 2019, 20(19), pp. 2479-2484. ISSN 1439-4227. eISSN 1439-7633. Available under: doi: 10.1002/cbic.201900318BibTex
@article{Kugele2019-10Prote-46050, year={2019}, doi={10.1002/cbic.201900318}, title={Protein Spin Labeling with a Photocaged Nitroxide Using Diels-Alder Chemistry}, number={19}, volume={20}, issn={1439-4227}, journal={Chembiochem}, pages={2479--2484}, author={Kugele, Anandi and Silkenath, Bjarne and Langer, Jakob and Wittmann, Valentin and Drescher, Malte} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/46050"> <dc:contributor>Silkenath, Bjarne</dc:contributor> <dc:creator>Silkenath, Bjarne</dc:creator> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/46050"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-06-19T11:47:28Z</dcterms:available> <dc:contributor>Drescher, Malte</dc:contributor> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/46050/1/Kugele_2-68onpzy40sdh1.pdf"/> <dc:rights>Attribution 4.0 International</dc:rights> <dc:language>eng</dc:language> <dc:creator>Wittmann, Valentin</dc:creator> <dcterms:issued>2019-10</dcterms:issued> <dc:creator>Kugele, Anandi</dc:creator> <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/> <dc:contributor>Wittmann, Valentin</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/46050/1/Kugele_2-68onpzy40sdh1.pdf"/> <dc:contributor>Kugele, Anandi</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2019-06-19T11:47:28Z</dc:date> <dc:creator>Langer, Jakob</dc:creator> <dcterms:title>Protein Spin Labeling with a Photocaged Nitroxide Using Diels-Alder Chemistry</dcterms:title> <dc:contributor>Langer, Jakob</dc:contributor> <dcterms:abstract xml:lang="eng">EPR spectroscopy of diamagnetic bio-macromolecules is based on site-directed spin labeling (SDSL). Here, we present a novel labeling strategy for proteins. We developed and synthesized a nitroxide-based spin label that can be ligated to proteins by an inverse-electron-demand Diels-Alder (DAinv) cycloaddition to genetically encoded non-canonical amino acids (ncAA). The nitroxide moiety is shielded by a photoremovable protecting group (PPG) with an attached tetraethylene glycol unit to achieve water solubility. We demonstrate SDSL of two model proteins with the PaNDA (Photoactivatable Nitroxide for DAinv reaction) label. Our strategy features high reaction rates combined with high selectivity, and the possibility to deprotect the nitroxide in E. coli lysate.</dcterms:abstract> <dc:creator>Drescher, Malte</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> </rdf:Description> </rdf:RDF>