Identification of glycoproteins specific to biotrophic intracellular hyphae formed in the Colletotrichum lindemuthianum-bean interaction

Pain, Naomi A.; O'Connell, Richard J.; Mendgen, Kurt; Green, Jonathan R.

Identification of glycoproteins specific to biotrophic intracellular hyphae formed in the Colletotrichum lindemuthianum-bean interaction

Dateien

Identification_of_glycoproteins_specific_to_biotrophic_intracellular_hyphae_formed_in_the_Colletotrichum_lindemuthianum_bean_interaction.pdfGröße: 4.41 MBDownloads: 316

Datum

1994

Autor:innen

Pain, Naomi A.

O'Connell, Richard J.

Mendgen, Kurt

Green, Jonathan R.

URI (zitierfähiger Link)

http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-34999

Link zur Lizenz

Attribution-NonCommercial-NoDerivs 2.0 Generic

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

New Phytologist. 1994, 127(2), pp. 233-242. ISSN 0028-646X. eISSN 1469-8137

Zusammenfassung

Monoclonal antibodies (MAbs) specific for intracellular hyphae (IH, i.e. infection vesicles and primary hyphae), appressoria/germ tubes and conidia of Colletotrichum lindemuthianum (Sacc. & Magn.) Briosi & Cav. isolated from infected leaves of Phaseolus vulgaris L. were obtained using a co-immunization procedure. One of the MAbs, UB25, bound specifically to IH in immunofluorescence, immunogold and Western blot assays: it showed no affinity for conidia, conodial germ tubes, appressoria or appressorial germ tubes growing in vitro, or for any plant components. Immunogold labelling of infected tissue prepared by high pressure freezing, freeze-substitution and low temperature embedding showed that the UB25 antigen was present in the interfacial matrix surrounding IH and in the fungal wall. The antigen was confined to infection vesicles and primary hyphae in contact with host protoplasts and could not be detected in primary hyphae growing in intercellular spaces. UB25 recognizes a protein epitope present in a set of N-linked glycoproteins. These glycoproteins are expressed at an early stage of intracellular development, suggesting a possible role in biotrophy or recognition.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Biotrophic pathogen, Colletotrichum lindemuthianum, high pressure freezing, intracellular hyphae, monoclonal antibody

Zitieren

ISO 690

PAIN, Naomi A., Richard J. O'CONNELL, Kurt MENDGEN, Jonathan R. GREEN, 1994. Identification of glycoproteins specific to biotrophic intracellular hyphae formed in the Colletotrichum lindemuthianum-bean interaction. In: New Phytologist. 1994, 127(2), pp. 233-242. ISSN 0028-646X. eISSN 1469-8137

BibTex

@article{Pain1994Ident-8301,
  year={1994},
  title={Identification of glycoproteins specific to biotrophic intracellular hyphae formed in the Colletotrichum lindemuthianum-bean interaction},
  number={2},
  volume={127},
  issn={0028-646X},
  journal={New Phytologist},
  pages={233--242},
  author={Pain, Naomi A. and O'Connell, Richard J. and Mendgen, Kurt and Green, Jonathan R.}
}

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Universitätsbibliographie

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