Crystallization and Preliminary X-Ray Diffraction Data of Two Different Human Low-Density Lipoprotein (LDL) Subfractions

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21_Ritter_ProtStrucFunctGen28_1997_293.pdf
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1997
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Ritter, Stephan
Frey, Ingrid
Grathwohl, Dominik
Keul, Joseph
Baumstark, Manfred W.
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Human LDL subfractions LDL-2 (d = 1.031 1.034 g/ml) and LDL-5 (d = 1.040 1.044 g/ml) were crystallized in two different crystal forms by using polyethylene glycol as a precipitant. Both fractions were from one donor. Crystals of LDL-5 were yellow, hexagonal, and showed no dichroism. Crystals of LDL-2 were of the same color, had a rodlike shape with notches at both ends, and were highly dichroitic. LDL-2 crystals diffracted to a resolution of 29 Å by using synchrotron radiation. Indexing in P1 resulted in preliminary parameters for the reduced cell of a = 171 Å, b = 438 Å, c = 519 Å, α = 102°, β = 99°, γ = 91. These dimensions are consistent with the size of LDL particles. Using Fourier transform infrared spectroscopy (FTIR) and agarose gel electrophoresis, we could further confirm that the crystals consist of LDL. The FTIR spectrum showed bands characteristic for lipids and protein. Dissolved crystals exhibited a mobility similar to native LDL in agarose gels and could be stained with anti-human apolipoprotein B (apoB).

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570 Biowissenschaften, Biologie
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crystallization, X-ray diffraction, human low-density lipoprotein subfractions
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ISO 690RITTER, Stephan, Ingrid FREY, Kay DIEDERICHS, Dominik GRATHWOHL, Joseph KEUL, Manfred W. BAUMSTARK, 1997. Crystallization and Preliminary X-Ray Diffraction Data of Two Different Human Low-Density Lipoprotein (LDL) Subfractions. In: Proteins: Structure, Function, and Genetics. 1997, 28(2), pp. 293-297. ISSN 0887-3585. eISSN 1097-0134. Available under: doi: 10.1002/(SICI)1097-0134(199706)28:2<293::AID-PROT17>3.0.CO;2-D
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@article{Ritter1997Cryst-7632,
  year={1997},
  doi={10.1002/(SICI)1097-0134(199706)28:2<293::AID-PROT17>3.0.CO;2-D},
  title={Crystallization and Preliminary X-Ray Diffraction Data of Two Different Human Low-Density Lipoprotein (LDL) Subfractions},
  number={2},
  volume={28},
  issn={0887-3585},
  journal={Proteins: Structure, Function, and Genetics},
  pages={293--297},
  author={Ritter, Stephan and Frey, Ingrid and Diederichs, Kay and Grathwohl, Dominik and Keul, Joseph and Baumstark, Manfred W.}
}
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