Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction

Hsp70 chaperones assist de novo folding of newly synthesized proteins in all cells. In yeast, the specialized Hsp70 Ssb directly binds to ribosomes. The structural basis and functional mode of recruitment of Ssb to ribosomes is not understood. Here, we present the molecular details underlying ribosome binding of Ssb in Saccharomyces cerevisiae. This interaction is multifaceted, involving the co-chaperone RAC and two specific regions within Ssb characterized by positive charges. The C-terminus of Ssb mediates the key contact and a second attachment point is provided by a KRR-motif in the substrate binding domain. Strikingly, ribosome binding of Ssb is not essential. Autonomous ribosome attachment becomes necessary if RAC is absent, suggesting a dual mode of Ssb recruitment to nascent chains. We propose, that the multilayered ribosomal interaction allows positioning of Ssb in an optimal orientation to the tunnel exit guaranteeing an efficient nascent polypeptide interaction.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

HANEBUTH, Marie A., Roman KITYK, Sandra J. FRIES, Alok JAIN, Allison KRIEL, Veronique ALBANESE, Tancred FRICKEY, Christine PETER, Matthias P. MAYER, Elke DEUERLING, 2016. Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction. In: Nature Communications. 2016, 7, 13695. eISSN 2041-1723. Available under: doi: 10.1038/ncomms13695

BibTex

@article{Hanebuth2016-12-05Multi-36508,
  year={2016},
  doi={10.1038/ncomms13695},
  title={Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction},
  volume={7},
  journal={Nature Communications},
  author={Hanebuth, Marie A. and Kityk, Roman and Fries, Sandra J. and Jain, Alok and Kriel, Allison and Albanese, Veronique and Frickey, Tancred and Peter, Christine and Mayer, Matthias P. and Deuerling, Elke},
  note={Article Number: 13695}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/36508">
    <dc:creator>Kriel, Allison</dc:creator>
    <dc:contributor>Mayer, Matthias P.</dc:contributor>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by/4.0/"/>
    <dc:contributor>Peter, Christine</dc:contributor>
    <dc:creator>Albanese, Veronique</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:creator>Deuerling, Elke</dc:creator>
    <dc:contributor>Frickey, Tancred</dc:contributor>
    <dc:contributor>Hanebuth, Marie A.</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/36508/3/Hanebuth_0-383854.pdf"/>
    <dc:creator>Kityk, Roman</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:creator>Peter, Christine</dc:creator>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/36508"/>
    <dc:contributor>Kriel, Allison</dc:contributor>
    <dc:contributor>Jain, Alok</dc:contributor>
    <dc:creator>Fries, Sandra J.</dc:creator>
    <dc:creator>Frickey, Tancred</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-01-05T09:07:50Z</dcterms:available>
    <dc:contributor>Albanese, Veronique</dc:contributor>
    <dc:language>eng</dc:language>
    <dc:contributor>Fries, Sandra J.</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Kityk, Roman</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:abstract xml:lang="eng">Hsp70 chaperones assist de novo folding of newly synthesized proteins in all cells. In yeast, the specialized Hsp70 Ssb directly binds to ribosomes. The structural basis and functional mode of recruitment of Ssb to ribosomes is not understood. Here, we present the molecular details underlying ribosome binding of Ssb in Saccharomyces cerevisiae. This interaction is multifaceted, involving the co-chaperone RAC and two specific regions within Ssb characterized by positive charges. The C-terminus of Ssb mediates the key contact and a second attachment point is provided by a KRR-motif in the substrate binding domain. Strikingly, ribosome binding of Ssb is not essential. Autonomous ribosome attachment becomes necessary if RAC is absent, suggesting a dual mode of Ssb recruitment to nascent chains. We propose, that the multilayered ribosomal interaction allows positioning of Ssb in an optimal orientation to the tunnel exit guaranteeing an efficient nascent polypeptide interaction.</dcterms:abstract>
    <dc:creator>Jain, Alok</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2017-01-05T09:07:50Z</dc:date>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/36508/3/Hanebuth_0-383854.pdf"/>
    <dc:rights>Attribution 4.0 International</dc:rights>
    <dc:creator>Mayer, Matthias P.</dc:creator>
    <dc:creator>Hanebuth, Marie A.</dc:creator>
    <dcterms:issued>2016-12-05</dcterms:issued>
    <dc:contributor>Deuerling, Elke</dc:contributor>
    <dcterms:title>Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction</dcterms:title>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Ja