Anaerobic aniline degradation via reductive deamination of 4-aminobenzoyl-CoA in Desulfobacterium anilini

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1991
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Schnell, Sylvia
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Archives of Microbiology. 1991, 155(2), pp. 183-190. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00248615
Zusammenfassung

The initial reactions involved in anaerobic aniline degradation by the sulfate-reducing Desulfobacterium anilini were studied. Experiments for substrate induction indicated the presence of a common pathway for aniline and 4-aminobenzoate, different from that for degradation of 2-aminobenzoate, 2-hydroxybenzoate, 4-hydroxybenzoate, or phenol. Degradation of aniline by dense cell suspensions depended on CO2 whereas 4-aminobenzoate degradation did not. If acetyl-CoA oxidation was inhibited by cyanide, benzoate accumulated during degradation of aniline or 4-aminobenzoate, indicating an initial carboxylation of aniline to 4-aminobenzoate, and further degradation via benzoate of both substrates. Extracts of aniline or 4-aminobenzoategrown cells activated 4-aminobenzoate to 4-aminobenzoyl-CoA in the presence of CoA, ATP and Mg 2+. 4-Aminobenzoyl-CoA-synthetase showed a Km for 4-aminobenzoate lower than 10 ~tM and an activity of 15.8 nmol - min- 1. mg- 1. 4-Aminobenzoyl-CoA was reductively deaminated to benzoyl-CoA by cell extracts in the presence of low-potential electron donors such as titanium citrate or cobalt sepulchrate (2.1 nmol - min -1 mg-1). Lower activities for the reductive deamination were measured with NADH or NADPH. Reductive deamination was also indicated by benzoate accumulation during 4-aminobenzoate degradation in cell suspensions under sulfate limitation. The results provide evidence that aniline is degraded via carboxylation to 4-aminobenzoate, which is activated to 4-aminobenzoyl-CoA and further metabolized by reductive deamination to benzoyl-CoA.

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Fachgebiet (DDC)
570 Biowissenschaften, Biologie
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Carboxylation, 4-Aminobenzoate, Benzoate, 4-Aminobenzoyl-CoA synthetase, 4-Aminobenzoyl, CoA reductase
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ISO 690SCHNELL, Sylvia, Bernhard SCHINK, 1991. Anaerobic aniline degradation via reductive deamination of 4-aminobenzoyl-CoA in Desulfobacterium anilini. In: Archives of Microbiology. 1991, 155(2), pp. 183-190. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00248615
BibTex
@article{Schnell1991Anaer-8330,
  year={1991},
  doi={10.1007/BF00248615},
  title={Anaerobic aniline degradation via reductive deamination of 4-aminobenzoyl-CoA in Desulfobacterium anilini},
  number={2},
  volume={155},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={183--190},
  author={Schnell, Sylvia and Schink, Bernhard}
}
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