Role of the Na+-translocating NADH:quinone oxidoreductase in voltage generation and Na+ extrusion in Vibrio cholerae

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2016
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Vorburger, Thomas
Bok, Eva
Schunke, Emina
Steffen, Wojtek
Götz, Friedrich
Steuber, Julia
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10.1016/j.bbabio.2015.12.010
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Biochimica et Biophysica Acta (BBA) - Bioenergetics ; 1857 (2016), 4. - pp. 473-482. - ISSN 0005-2728. - eISSN 1879-2650
Abstract
For Vibrio cholerae, the coordinated import and export of Na+ is crucial for adaptation to habitats with different osmolarities. We investigated the Na+-extruding branch of the sodium cycle in this human pathogen by in vivo (23)Na-NMR spectroscopy. The Na+ extrusion activity of cells was monitored after adding glucose which stimulated respiration via the Na+-translocating NADH:quinone oxidoreductase (Na(+)-NQR). In a V. cholerae deletion mutant devoid of the Na+-NQR encoding genes (nqrA-F), rates of respiratory Na+ extrusion were decreased by a factor of four, but the cytoplasmic Na(+) concentration was essentially unchanged. Furthermore, the mutant was impaired in formation of transmembrane voltage (ΔΨ, inside negative) and did not grow under hypoosmotic conditions at pH8.2 or above. This growth defect could be complemented by transformation with the plasmid encoded nqr operon. In an alkaline environment, Na+/H+ antiporters acidify the cytoplasm at the expense of the transmembrane voltage. It is proposed that, at alkaline pH and limiting Na+ concentrations, the Na+-NQR is crucial for generation of a transmembrane voltage to drive the import of H(+) by electrogenic Na+/H+ antiporters. Our study provides the basis to understand the role of the Na+-NQR in pathogenicity of V. cholerae and other pathogens relying on this primary Na+ pump for respiration.
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ISO 690VORBURGER, Thomas, Ruslan NEDIELKOV, Alexander BROSIG, Eva BOK, Emina SCHUNKE, Wojtek STEFFEN, Sonja MAYER, Friedrich GÖTZ, Heiko M. MÖLLER, Julia STEUBER, 2016. Role of the Na+-translocating NADH:quinone oxidoreductase in voltage generation and Na+ extrusion in Vibrio cholerae. In: Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1857(4), pp. 473-482. ISSN 0005-2728. eISSN 1879-2650. Available under: doi: 10.1016/j.bbabio.2015.12.010
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@article{Vorburger2016trans-34019,
  year={2016},
  doi={10.1016/j.bbabio.2015.12.010},
  title={Role of the Na<sup>+</sup>-translocating NADH:quinone oxidoreductase in voltage generation and Na+ extrusion in Vibrio cholerae},
  number={4},
  volume={1857},
  issn={0005-2728},
  journal={Biochimica et Biophysica Acta (BBA) - Bioenergetics},
  pages={473--482},
  author={Vorburger, Thomas and Nedielkov, Ruslan and Brosig, Alexander and Bok, Eva and Schunke, Emina and Steffen, Wojtek and Mayer, Sonja and Götz, Friedrich and Möller, Heiko M. and Steuber, Julia}
}
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