Engineering of a DNA Polymerase for Direct m6A Sequencing
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2018
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Werner, Stephan
Marchand, Virginie
Motorin, Yuri
Helm, Mark
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Angewandte Chemie International Edition. 2018, 57(2), pp. 417-421. ISSN 0570-0833. eISSN 1521-3773. Available under: doi: 10.1002/anie.201710209
Zusammenfassung
Methods for the detection of RNA modifications are of fundamental importance for advancing epitranscriptomics. N6-methyladenosine (m6A) is the most abundant RNA modification in mammalian mRNA and is involved in the regulation of gene expression. Current detection techniques are laborious and rely on antibody-based enrichment of m6A-containing RNA prior to sequencing, since m6A modifications are generally "erased" during reverse transcription (RT). To overcome the drawbacks associated with indirect detection, we aimed to generate novel DNA polymerase variants for direct m6A sequencing. Therefore, we developed a screen to evolve an RT-active KlenTaq DNA polymerase variant that sets a mark for N6-methylation. We identified a mutant that exhibits increased misincorporation opposite m6A compared to unmodified A. Application of the generated DNA polymerase in next-generation sequencing allowed the identification of m6A sites directly from the sequencing data of untreated RNA samples.
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ASCHENBRENNER, Joos, Stephan WERNER, Virginie MARCHAND, Martina ADAM, Yuri MOTORIN, Mark HELM, Andreas MARX, 2018. Engineering of a DNA Polymerase for Direct m6A Sequencing. In: Angewandte Chemie International Edition. 2018, 57(2), pp. 417-421. ISSN 0570-0833. eISSN 1521-3773. Available under: doi: 10.1002/anie.201710209BibTex
@article{Aschenbrenner2018-01-08Engin-41793,
year={2018},
doi={10.1002/anie.201710209},
title={Engineering of a DNA Polymerase for Direct m<sup>6</sup>A Sequencing},
number={2},
volume={57},
issn={0570-0833},
journal={Angewandte Chemie International Edition},
pages={417--421},
author={Aschenbrenner, Joos and Werner, Stephan and Marchand, Virginie and Adam, Martina and Motorin, Yuri and Helm, Mark and Marx, Andreas}
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<dcterms:abstract xml:lang="eng">Methods for the detection of RNA modifications are of fundamental importance for advancing epitranscriptomics. N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) is the most abundant RNA modification in mammalian mRNA and is involved in the regulation of gene expression. Current detection techniques are laborious and rely on antibody-based enrichment of m<sup>6</sup>A-containing RNA prior to sequencing, since m<sup>6</sup>A modifications are generally "erased" during reverse transcription (RT). To overcome the drawbacks associated with indirect detection, we aimed to generate novel DNA polymerase variants for direct m<sup>6</sup>A sequencing. Therefore, we developed a screen to evolve an RT-active KlenTaq DNA polymerase variant that sets a mark for N<sup>6</sup>-methylation. We identified a mutant that exhibits increased misincorporation opposite m<sup>6</sup>A compared to unmodified A. Application of the generated DNA polymerase in next-generation sequencing allowed the identification of m<sup>6</sup>A sites directly from the sequencing data of untreated RNA samples.</dcterms:abstract>
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