Effect of khat chewing on periodontal pathogens in subgingival biofilm from chronic periodontitis patients
Effect of khat chewing on periodontal pathogens in subgingival biofilm from chronic periodontitis patients
Loading...
Date
2010
Authors
Al-Hebshi, Nezar Noor
Al-Sharabi, Ali Kaid
Shuga-Aldin, Hussein Mohammed
Al-Haroni, Mohammed
Ghandour, Ibrahim
Editors
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
URI (citable link)
DOI (citable link)
International patent number
Link to the license
EU project number
Project
Open Access publication
Collections
Title in another language
Publication type
Journal article
Publication status
Published in
Journal of Ethnopharmacology ; 132 (2010), 3. - pp. 564-569
Abstract
Aims: Existing in vitro and in vivo data suggest that khat may have a favorable effect on periodontal microbiota. The purpose of this study was to assess the effect of khat chewing on major periodontal pathogens in subgingival plaque samples from subjects with chronic periodontitis.
Materials and methods: 40 subgingival plaque samples were obtained from periodontitis and healthy sites of 10 khat chewers (40 y median age) and 10 khat non-chewers (37.5 y median age) with chronic periodontitis. Absolute and relative counts of 6 periodontal pathogens were determined in each sample using highly sensitive and specific Taqman real-time PCR assays. Data were analyzed using an ordinal regression model.
Results: Significantly more total bacteria were detected in samples from the periodontitis sites of the khat chewers (OR = 20). Treponema denticola was present at significantly higher absolute counts at the healthy as well as periodontitis sites of the khat chewers (OR = 3.13 and 13, respectively). However, the khat chewers harbored significantly lower absolute counts of Porphyromonas gingivalis at the healthy sites
(OR = 0.07). Furthermore, khat chewing was significantly associated with lower relative counts of Porphyromonas gingivalis, fusobacterium ssp., prevotella ssp. and Parvimonas micra-like species in subgingival plaque samples from both healthy and periodontitis sites (OR = 0.11 0.33). Only Treponema denticola was found in higher relative counts at the healthy sites of the khat chewers (OR = 2.98).
Conclusions: Overall, there was a lower burden of pathogens in the khat chewers. Findings from the current study are suggestive of a potential prebiotic effect for khat on periodontal microbiota.
Materials and methods: 40 subgingival plaque samples were obtained from periodontitis and healthy sites of 10 khat chewers (40 y median age) and 10 khat non-chewers (37.5 y median age) with chronic periodontitis. Absolute and relative counts of 6 periodontal pathogens were determined in each sample using highly sensitive and specific Taqman real-time PCR assays. Data were analyzed using an ordinal regression model.
Results: Significantly more total bacteria were detected in samples from the periodontitis sites of the khat chewers (OR = 20). Treponema denticola was present at significantly higher absolute counts at the healthy as well as periodontitis sites of the khat chewers (OR = 3.13 and 13, respectively). However, the khat chewers harbored significantly lower absolute counts of Porphyromonas gingivalis at the healthy sites
(OR = 0.07). Furthermore, khat chewing was significantly associated with lower relative counts of Porphyromonas gingivalis, fusobacterium ssp., prevotella ssp. and Parvimonas micra-like species in subgingival plaque samples from both healthy and periodontitis sites (OR = 0.11 0.33). Only Treponema denticola was found in higher relative counts at the healthy sites of the khat chewers (OR = 2.98).
Conclusions: Overall, there was a lower burden of pathogens in the khat chewers. Findings from the current study are suggestive of a potential prebiotic effect for khat on periodontal microbiota.
Summary in another language
Subject (DDC)
150 Psychology
Keywords
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690
AL-HEBSHI, Nezar Noor, Ali Kaid AL-SHARABI, Hussein Mohammed SHUGA-ALDIN, Mohammed AL-HARONI, Ibrahim GHANDOUR, 2010. Effect of khat chewing on periodontal pathogens in subgingival biofilm from chronic periodontitis patients. In: Journal of Ethnopharmacology. 132(3), pp. 564-569. Available under: doi: 10.1016/j.jep.2010.08.051BibTex
@article{AlHebshi2010Effec-10815, year={2010}, doi={10.1016/j.jep.2010.08.051}, title={Effect of khat chewing on periodontal pathogens in subgingival biofilm from chronic periodontitis patients}, number={3}, volume={132}, journal={Journal of Ethnopharmacology}, pages={564--569}, author={Al-Hebshi, Nezar Noor and Al-Sharabi, Ali Kaid and Shuga-Aldin, Hussein Mohammed and Al-Haroni, Mohammed and Ghandour, Ibrahim} }
RDF
<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/10815"> <dcterms:title>Effect of khat chewing on periodontal pathogens in subgingival biofilm from chronic periodontitis patients</dcterms:title> <dc:creator>Al-Haroni, Mohammed</dc:creator> <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/> <dc:creator>Shuga-Aldin, Hussein Mohammed</dc:creator> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:format>application/pdf</dc:format> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/10815"/> <dcterms:bibliographicCitation>First publ. in: Journal of Ethnopharmacology 132 (2010), 3. pp. 564-569</dcterms:bibliographicCitation> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/43"/> <dc:creator>Al-Sharabi, Ali Kaid</dc:creator> <dc:contributor>Shuga-Aldin, Hussein Mohammed</dc:contributor> <dc:contributor>Al-Hebshi, Nezar Noor</dc:contributor> <dc:language>eng</dc:language> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/10815/1/JEP_6308.pdf"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-25T09:22:53Z</dc:date> <foaf:homepage rdf:resource="http://localhost:8080/"/> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/43"/> <dc:contributor>Ghandour, Ibrahim</dc:contributor> <dc:contributor>Al-Haroni, Mohammed</dc:contributor> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/10815/1/JEP_6308.pdf"/> <dc:creator>Ghandour, Ibrahim</dc:creator> <dc:contributor>Al-Sharabi, Ali Kaid</dc:contributor> <dcterms:abstract xml:lang="eng">Aims: Existing in vitro and in vivo data suggest that khat may have a favorable effect on periodontal microbiota. The purpose of this study was to assess the effect of khat chewing on major periodontal pathogens in subgingival plaque samples from subjects with chronic periodontitis.<br />Materials and methods: 40 subgingival plaque samples were obtained from periodontitis and healthy sites of 10 khat chewers (40 y median age) and 10 khat non-chewers (37.5 y median age) with chronic periodontitis. Absolute and relative counts of 6 periodontal pathogens were determined in each sample using highly sensitive and specific Taqman real-time PCR assays. Data were analyzed using an ordinal regression model.<br />Results: Significantly more total bacteria were detected in samples from the periodontitis sites of the khat chewers (OR = 20). Treponema denticola was present at significantly higher absolute counts at the healthy as well as periodontitis sites of the khat chewers (OR = 3.13 and 13, respectively). However, the khat chewers harbored significantly lower absolute counts of Porphyromonas gingivalis at the healthy sites<br />(OR = 0.07). Furthermore, khat chewing was significantly associated with lower relative counts of Porphyromonas gingivalis, fusobacterium ssp., prevotella ssp. and Parvimonas micra-like species in subgingival plaque samples from both healthy and periodontitis sites (OR = 0.11 0.33). Only Treponema denticola was found in higher relative counts at the healthy sites of the khat chewers (OR = 2.98).<br />Conclusions: Overall, there was a lower burden of pathogens in the khat chewers. Findings from the current study are suggestive of a potential prebiotic effect for khat on periodontal microbiota.</dcterms:abstract> <dc:rights>terms-of-use</dc:rights> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-25T09:22:53Z</dcterms:available> <dcterms:issued>2010</dcterms:issued> <dc:creator>Al-Hebshi, Nezar Noor</dc:creator> </rdf:Description> </rdf:RDF>