Esterases in the zebra mussel Dreissena polymorpha : activities, inhibition, and binding to organophosphates

Lade...
Vorschaubild
Dateien
Datum
1997
Autor:innen
Dauberschmidt, Carole
Schlatter, Christian
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Aquatic Toxicology. 1997, 37(4), pp. 295-305. ISSN 0166-445X. Available under: doi: 10.1016/S0166-445X(96)00831-4
Zusammenfassung

Cholinesterase activities of Dreissena polymorpha were measured colorimetrically. In homogenates of whole control mussel, activities of 125 ± 29 μmol min -1 kg -1 were found (n = 6). Neither after exposure of Dreissena to organophosphates (thiometon, disulfoton, demeton-S-methyl) nor after addition of demeton-S-methyl (the activated oxygen analogue of thiometon) in vitro was the measured mussel esterase activity inhibited. Esterases of rat, mouse and human tissue showed a 90 100% inhibition. Radiolabelling of the active serine site of esterases in muscle homogenates with 3H-diisopropylfluorophosphate and subsequent separation on polyacrylamide gels revealed similarities as well as differences between rat and mussel esterases. Coomassie-stained muscle proteins of Dreissena showed a different distribution pattern than those of rat. Proteins of rat as well as proteins of mussel with molecular weights between 66 and 97 kDa showed best labelling (highest radioactivity). Proteins with molecular weights greater than 97 kDa were not labelled. Additionally, in Dreissena but not in rat, proteins of around 45 kDa were labelled. The results indicate that the esteratic enzymes in Dreissena were labelled but not inhibited by organophosphates.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Dreissena polymorpha, Organophosphate, Acetylcholinesterase, Radiolabelling, Diisopropylfluorophosphate
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690DAUBERSCHMIDT, Carole, Daniel R. DIETRICH, Christian SCHLATTER, 1997. Esterases in the zebra mussel Dreissena polymorpha : activities, inhibition, and binding to organophosphates. In: Aquatic Toxicology. 1997, 37(4), pp. 295-305. ISSN 0166-445X. Available under: doi: 10.1016/S0166-445X(96)00831-4
BibTex
@article{Dauberschmidt1997Ester-7485,
  year={1997},
  doi={10.1016/S0166-445X(96)00831-4},
  title={Esterases in the zebra mussel Dreissena polymorpha : activities, inhibition, and binding to organophosphates},
  number={4},
  volume={37},
  issn={0166-445X},
  journal={Aquatic Toxicology},
  pages={295--305},
  author={Dauberschmidt, Carole and Dietrich, Daniel R. and Schlatter, Christian}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7485">
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7485/1/Esterases_in_the_zebra_mussel_Dreissena_polymorpha_.pdf"/>
    <dc:contributor>Dauberschmidt, Carole</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7485"/>
    <dc:contributor>Schlatter, Christian</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:abstract xml:lang="deu">Cholinesterase activities of Dreissena polymorpha were measured colorimetrically. In homogenates of whole control mussel, activities of 125 ± 29 μmol min -1 kg -1 were found (n = 6). Neither after exposure of Dreissena to organophosphates (thiometon, disulfoton, demeton-S-methyl) nor after addition of demeton-S-methyl (the activated oxygen analogue of thiometon) in vitro was the measured mussel esterase activity inhibited. Esterases of rat, mouse and human tissue showed a 90 100% inhibition. Radiolabelling of the active serine site of esterases in muscle homogenates with 3H-diisopropylfluorophosphate and subsequent separation on polyacrylamide gels revealed similarities as well as differences between rat and mussel esterases. Coomassie-stained muscle proteins of Dreissena showed a different distribution pattern than those of rat. Proteins of rat as well as proteins of mussel with molecular weights between 66 and 97 kDa showed best labelling (highest radioactivity). Proteins with molecular weights greater than 97 kDa were not labelled. Additionally, in Dreissena but not in rat, proteins of around 45 kDa were labelled. The results indicate that the esteratic enzymes in Dreissena were labelled but not inhibited by organophosphates.</dcterms:abstract>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:34:48Z</dc:date>
    <dcterms:issued>1997</dcterms:issued>
    <dc:language>eng</dc:language>
    <dc:creator>Schlatter, Christian</dc:creator>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:title>Esterases in the zebra mussel Dreissena polymorpha : activities, inhibition, and binding to organophosphates</dcterms:title>
    <dcterms:bibliographicCitation>First publ. in: Aquatic Toxicology 37 (1997), 4, pp. 295-305</dcterms:bibliographicCitation>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:34:48Z</dcterms:available>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dc:format>application/pdf</dc:format>
    <dc:contributor>Dietrich, Daniel R.</dc:contributor>
    <dc:creator>Dauberschmidt, Carole</dc:creator>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7485/1/Esterases_in_the_zebra_mussel_Dreissena_polymorpha_.pdf"/>
    <dc:creator>Dietrich, Daniel R.</dc:creator>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen