Perlecan and Dystroglycan act at the basal side of the Drosophila follicular epithelium to maintain epithelial organization

Schneider, Martina; Khalil, Ashraf A.; Poulton, John; Castillejo-Lopez, Casimiro; Egger-Adam, Diane; Wodarz, Andreas; Deng, Wu-Min; Baumgartner, Stefan

Perlecan and Dystroglycan act at the basal side of the Drosophila follicular epithelium to maintain epithelial organization

Dateien

Zu diesem Dokument gibt es keine Dateien.

Datum

2006

Autor:innen

Schneider, Martina

Khalil, Ashraf A.

Poulton, John

Castillejo-Lopez, Casimiro

Egger-Adam, Diane

Wodarz, Andreas

Deng, Wu-Min

Baumgartner, Stefan

DOI (zitierfähiger Link)

https://doi.org/10.1242/dev.02549

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Development. Company of Biologists. 2006, 133(19), pp. 3805-3815. ISSN 0950-1991. eISSN 1477-9129. Available under: doi: 10.1242/dev.02549

Zusammenfassung

Dystroglycan (Dg) is a widely expressed extracellular matrix (ECM) receptor required for muscle viability, synaptogenesis, basementmembrane formation and epithelial development. As an integral component of the Dystrophin-associated glycoprotein complex, Dg plays a central role in linking the ECM and the cytoskeleton. Disruption of this linkage in skeletal muscle leads to various types of muscular dystrophies. In epithelial cells, reduced expression of Dg is associated with increased invasiveness of cancer cells. We have previously shown that Dg is required for epithelial cell polarity in Drosophila, but the mechanisms of this polarizing activity and upstream/downstream components are largely unknown. Using the Drosophila follicle-cell epithelium (FCE) as a model system, we show that the ECM molecule Perlecan (Pcan) is required for maintenance of epithelial-cell polarity. Follicle cells that lack Pcan develop polarity defects similar to those of Dg mutant cells. Furthermore, Dg depends on Pcan but not on Laminin A for its localization in the basal-cell membrane, and the two proteins bind in vitro. Interestingly, the Dg form that interacts with Pcan in the FCE lacks the mucin-like domain, which is thought to be essential for Dg ligand binding activity. Finally, we describe two examples of how Dg promotes the differentiation of the basal membrane domain: (1) by recruiting/anchoring the cytoplasmic protein Dystrophin; and (2) by excluding the transmembrane protein Neurexin. We suggest that the interaction of Pcan and Dg at the basal side of the epithelium promotes basal membrane differentiation and is required for maintenance of cell polarity in the FCE.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

Perlecan, Dystroglycan, Laminin, Dystrophin, Neurexin, Polarity, Epithelia, Oogenesis, Drosophila

Zitieren

ISO 690

SCHNEIDER, Martina, Ashraf A. KHALIL, John POULTON, Casimiro CASTILLEJO-LOPEZ, Diane EGGER-ADAM, Andreas WODARZ, Wu-Min DENG, Stefan BAUMGARTNER, 2006. Perlecan and Dystroglycan act at the basal side of the Drosophila follicular epithelium to maintain epithelial organization. In: Development. Company of Biologists. 2006, 133(19), pp. 3805-3815. ISSN 0950-1991. eISSN 1477-9129. Available under: doi: 10.1242/dev.02549

BibTex

@article{Schneider2006-10Perle-50887,
  year={2006},
  doi={10.1242/dev.02549},
  title={Perlecan and Dystroglycan act at the basal side of the Drosophila follicular epithelium to maintain epithelial organization},
  number={19},
  volume={133},
  issn={0950-1991},
  journal={Development},
  pages={3805--3815},
  author={Schneider, Martina and Khalil, Ashraf A. and Poulton, John and Castillejo-Lopez, Casimiro and Egger-Adam, Diane and Wodarz, Andreas and Deng, Wu-Min and Baumgartner, Stefan}
}

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Universitätsbibliographie

Ja

Begutachtet

Ja