Intracellular tracking of single-plasmid DNA particles after delivery by electroporation

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Electroporation is a physical method of transferring molecules into cells and tissues. It takes advantage of the transient permeabilization of the cell membrane induced by electric field pulses, which gives hydrophilic molecules access to the cytoplasm. This method offers high transfer efficiency for small molecules that freely diffuse though electrically permeabilized membrane. Larger molecules, such as plasmid DNA, face several barriers (plasma membrane, cytoplasmic crowding, nuclear envelope) which reduce transfection efficiency and engender a complex mechanism of transfer. Our work provides insight into the way electrotransferred DNA crosses the cytoplasm to reach the nucleus. For this purpose, single particle tracking experiments of fluorescently labeled DNA were performed. Investigations were focused on the involvement of the cytoskeleton using drugs disrupting or stabilizing actin and tubulin filaments as the two relevant cellular networks for particle transport. The analysis of 315 movies (~ 4000 trajectories) reveals that DNA is actively transported via the cytoskeleton. The large number of events allows a statistical quantification of the DNA motion kinetics inside the cell. Disruption of both filament types reduces active transport occurrence, velocities, and displacements of DNA particles. Interestingly, stabilization of both networks does not enhance DNA transport.

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ISO 690ROSAZZA, Christelle, Annette BUNTZ, Thorsten RIESS, Dominik WÖLL, Andreas ZUMBUSCH, Marie-Pierre ROLS, 2013. Intracellular tracking of single-plasmid DNA particles after delivery by electroporation. In: Molecular Therapy. 2013, 21(12), pp. 2217-2226. ISSN 1525-0016. eISSN 1525-0024. Available under: doi: 10.1038/mt.2013.182
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@article{Rosazza2013-12Intra-24502,
  year={2013},
  doi={10.1038/mt.2013.182},
  title={Intracellular tracking of single-plasmid DNA particles after delivery by electroporation},
  number={12},
  volume={21},
  issn={1525-0016},
  journal={Molecular Therapy},
  pages={2217--2226},
  author={Rosazza, Christelle and Buntz, Annette and Rieß, Thorsten and Wöll, Dominik and Zumbusch, Andreas and Rols, Marie-Pierre}
}
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