@incollection{Rutschlin2020Engin-50650,
  year={2020},
  doi={10.1016/bs.mie.2019.10.030},
  title={Engineering siderophores},
  number={633},
  isbn={978-0-12-819128-6},
  publisher={Elsevier},
  address={Cambridge, MA},
  series={Methods in Enzymology},
  booktitle={Chemical and synthetic biology approaches to understand cellular functions : part C},
  pages={29--47},
  editor={Shukla, Arun K.},
  author={Rütschlin, Sina and Böttcher, Thomas}
}

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    <dc:contributor>Böttcher, Thomas</dc:contributor>
    <dc:creator>Böttcher, Thomas</dc:creator>
    <dc:creator>Rütschlin, Sina</dc:creator>
    <dcterms:abstract xml:lang="eng">Siderophores have important functions for bacteria in iron acquisition and as virulence factors. In this chapter we will discuss the engineering of cyclic hydroxamate siderophores by various biochemical approaches based on the example of Shewanella algae. The marine gamma-proteobacterium S. algae produces three different cyclic hydroxamate siderophores as metabolites via a single biosynthetic gene cluster and one of them is an important key player in interspecies competition blocking swarming of Vibrio alginolyticus. AvbD is the key metabolic enzyme assembling the precursors into three different core structures and hence an interesting target for metabolic and biochemical engineering. Synthetic natural and unnatural precursors can be converted in vitro with purified AvbD to generate siderophores with various ring sizes ranging from analytical to milligram scale. These engineered siderophores can be applied, for example, as swarming inhibitors against V. alginolyticus. Here, we describe the synthesis of the natural and unnatural siderophore precursors HS[X]A and provide our detailed protocols for protein expression of AvbD, conversion of HS[X]A with the enzyme to produce ring-size engineered siderophores and secondly for a biosynthetic feeding strategy that allows to extract engineered siderophores in the milligram scale.</dcterms:abstract>
    <dcterms:issued>2020</dcterms:issued>
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    <dcterms:title>Engineering siderophores</dcterms:title>
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    <dc:contributor>Rütschlin, Sina</dc:contributor>
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