Publikation:

A five-gene cluster involved in utilization of taurine-nitrogen and excretion of sulfoacetaldehyde by Acinetobacter radioresistens SH164

Vorschaubild

Dateien

cluster_schleheck_etal.pdf
cluster_schleheck_etal.pdfGröße: 4.97 MBDownloads: 1007

Datum

2012

Autor:innen

Krejčík, Zdenĕk
Hollemeyer, Klaus

Herausgeber:innen

Kontakt

ISSN der Zeitschrift

Electronic ISSN

ISBN

Bibliografische Daten

Verlag

Schriftenreihe

Auflagebezeichnung

URI (zitierfähiger Link)
http://nbn-resolving.de/urn:nbn:de:bsz:352-193757
DOI (zitierfähiger Link)
https://doi.org/10.1007/s00203-012-0806-1
ArXiv-ID

Internationale Patentnummer

Link zur Lizenz
Urheberrechtlich geschützt

Angaben zur Forschungsförderung

Projekt

Open Access-Veröffentlichung
Open Access Green

Sammlungen

Biologie: Publikationen
Core Facility der Universität Konstanz

Gesperrt bis

Titel in einer weiteren Sprache

Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published

Erschienen in

Archives of Microbiology. 2012, 194(10), pp. 857-863. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s00203-012-0806-1

Zusammenfassung

Acinetobacter calcoaceticus SW1, under nitrogen limitation, assimilates the nitrogen moiety of taurine (2-aminoethanesulfonate) inducibly and excretes sulfoacetaldehyde, a product of taurine dehydrogenase (TauXY). BLAST searches of newly available genome sequences using the TauXY sequences revealed a 5-gene cluster, tauRXYPI, in Acinetobacter radioresistens SH164. We hypothesized that tauXYPI (HMPREF0018_00717-HMPREF0018_00720) encodes proteins that are orthologs of the undefined pathway from strain SW1, and that tauR (HMPREF0018_00716) encodes the relevant transcriptional regulator. Strain SH164 excreted sulfoacetaldehyde from taurine during growth. TauXY activity was expressed inducibly. Reverse transcription PCR showed that the tauRXYPI genes were transcribed inducibly. This allowed the conclusions that (i) TauP (currently annotated as permease GabP [TC 2.A.3]) is a taurine permease, and (ii) TauI (currently annotated as DUF6 drug/metabolite exporter [TC 2.A.7]) is a sulfoacetaldehyde exporter. The presumably equifunctional cluster tauRXYPI was then found in strain SW1. TauP is the third recognized taurine uptake system, and TauI is the third postulated class of sulfonate exporters, in bacteria.

Zusammenfassung in einer weiteren Sprache

Fachgebiet (DDC)
570 Biowissenschaften, Biologie

Schlagwörter

Konferenz

Rezension
undefined / . - undefined, undefined

Forschungsvorhaben

Organisationseinheiten

Zeitschriftenheft

Zugehörige Datensätze in KOPS

Zitieren

ISO 690KREJČÍK, Zdenĕk, David SCHLEHECK, Klaus HOLLEMEYER, Alasdair M. COOK, 2012. A five-gene cluster involved in utilization of taurine-nitrogen and excretion of sulfoacetaldehyde by Acinetobacter radioresistens SH164. In: Archives of Microbiology. 2012, 194(10), pp. 857-863. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/s00203-012-0806-1
BibTex
@article{Krejcik2012-10fiveg-19375,
  year={2012},
  doi={10.1007/s00203-012-0806-1},
  title={A five-gene cluster involved in utilization of taurine-nitrogen and excretion of sulfoacetaldehyde by Acinetobacter radioresistens SH164},
  number={10},
  volume={194},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={857--863},
  author={Krejčík, Zdenĕk and Schleheck, David and Hollemeyer, Klaus and Cook, Alasdair M.}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/19375">
    <dcterms:title>A five-gene cluster involved in utilization of taurine-nitrogen and excretion of sulfoacetaldehyde by Acinetobacter radioresistens SH164</dcterms:title>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Schleheck, David</dc:contributor>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/19375/2/cluster_schleheck_etal.pdf"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2013-10-31T23:25:04Z</dcterms:available>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/19375/2/cluster_schleheck_etal.pdf"/>
    <dc:contributor>Krejčík, Zdenĕk</dc:contributor>
    <dc:creator>Hollemeyer, Klaus</dc:creator>
    <dcterms:bibliographicCitation>Archives of Microbiology ; 194 (2012), 10. - S. 857-869</dcterms:bibliographicCitation>
    <dc:creator>Cook, Alasdair M.</dc:creator>
    <dcterms:issued>2012-10</dcterms:issued>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Hollemeyer, Klaus</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/19375"/>
    <dc:creator>Schleheck, David</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2012-09-25T12:35:07Z</dc:date>
    <dc:creator>Krejčík, Zdenĕk</dc:creator>
    <dc:language>eng</dc:language>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Cook, Alasdair M.</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:abstract>Acinetobacter calcoaceticus SW1, under nitrogen limitation, assimilates the nitrogen moiety of taurine (2-aminoethanesulfonate) inducibly and excretes sulfoacetaldehyde, a product of taurine dehydrogenase (TauXY). BLAST searches of newly available genome sequences using the TauXY sequences revealed a 5-gene cluster, tauRXYPI, in Acinetobacter radioresistens SH164. We hypothesized that tauXYPI (HMPREF0018_00717-HMPREF0018_00720) encodes proteins that are orthologs of the undefined pathway from strain SW1, and that tauR (HMPREF0018_00716) encodes the relevant transcriptional regulator. Strain SH164 excreted sulfoacetaldehyde from taurine during growth. TauXY activity was expressed inducibly. Reverse transcription PCR showed that the tauRXYPI genes were transcribed inducibly. This allowed the conclusions that (i) TauP (currently annotated as permease GabP [TC 2.A.3]) is a taurine permease, and (ii) TauI (currently annotated as DUF6 drug/metabolite exporter [TC 2.A.7]) is a sulfoacetaldehyde exporter. The presumably equifunctional cluster tauRXYPI was then found in strain SW1. TauP is the third recognized taurine uptake system, and TauI is the third postulated class of sulfonate exporters, in bacteria.</dcterms:abstract>
  </rdf:Description>
</rdf:RDF>

Interner Vermerk

xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter

Kontakt
URL der Originalveröffentl.

Prüfdatum der URL

Prüfungsdatum der Dissertation

Finanzierungsart

Kommentar zur Publikation

Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen