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Impact of Cellular Crowding on Protein Structural Dynamics Investigated by EPR Spectroscopy

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2024

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Bonucci, Alessio
Magalon, Axel
Belle, Valérie
Mileo, Elisabetta

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European Union (EU): 101004806

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Chemical Reviews. ACS Publications. 2024, 124(17), S. 9873-9898. ISSN 0009-2665. eISSN 1520-6890. Verfügbar unter: doi: 10.1021/acs.chemrev.3c00951

Zusammenfassung

The study of how the intracellular medium influences protein structural dynamics and protein–protein interactions is a captivating area of research for scientists aiming to comprehend biomolecules in their native environment. As the cellular environment can hardly be reproduced in vitro, direct investigation of biomolecules within cells has attracted growing interest in the past two decades. Among magnetic resonances, site-directed spin labeling coupled to electron paramagnetic resonance spectroscopy (SDSL-EPR) has emerged as a powerful tool for studying the structural properties of biomolecules directly in cells. Since the first in-cell EPR experiment was reported in 2010, substantial progress has been made, and this Review provides a detailed overview of the developments and applications of this spectroscopic technique. The strategies available for preparing a cellular sample and the EPR methods that can be applied to cells will be discussed. The array of spin labels available, along with their strengths and weaknesses in cellular contexts, will also be described. Several examples will illustrate how in-cell EPR can be applied to different biological systems and how the cellular environment affects the structural and dynamic properties of different proteins. Lastly, the Review will focus on the future developments expected to expand the capabilities of this promising technique.

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540 Chemie

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ISO 690PIERRO, Annalisa, Alessio BONUCCI, Axel MAGALON, Valérie BELLE, Elisabetta MILEO, 2024. Impact of Cellular Crowding on Protein Structural Dynamics Investigated by EPR Spectroscopy. In: Chemical Reviews. ACS Publications. 2024, 124(17), S. 9873-9898. ISSN 0009-2665. eISSN 1520-6890. Verfügbar unter: doi: 10.1021/acs.chemrev.3c00951
BibTex
@article{Pierro2024-09-11Impac-70764,
  year={2024},
  doi={10.1021/acs.chemrev.3c00951},
  title={Impact of Cellular Crowding on Protein Structural Dynamics Investigated by EPR Spectroscopy},
  number={17},
  volume={124},
  issn={0009-2665},
  journal={Chemical Reviews},
  pages={9873--9898},
  author={Pierro, Annalisa and Bonucci, Alessio and Magalon, Axel and Belle, Valérie and Mileo, Elisabetta}
}
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