Purification of the yellow fluorescent protein from vibrio fischeri and identity of the flavin chromophore

Lade...
Vorschaubild
Datum
1987
Autor:innen
Macheroux, Peter
Schmidt, K. U.
Steinerstauch, Petra
Ghisla, Sandro
Colepicolo, Pio
Buntic, Rudolf
Hastings, J. Woodland
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
DOI (zitierfähiger Link)
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Biochemical and Biophysical Research Communications. 1987, 146(1), pp. 101-106. ISSN 0006-291X
Zusammenfassung

A low molecular weight protein (~ 25,000 D) exhibiting a yellow fluorescence emission peaking at ~ 540 nm was isolated from Vibrio fischeri (strain Y-1) and purified to apparent homogeneity. FMN is the chromophore, but it exhibits marked red shifts in both the absorption (λmax = 380, 460 nm) and the fluorescence emission. When added to purified luciferase from the same strain, which itself catalyzes an emission of blue-green light (λmax ~ 495 nm), this protein induces a bright yellow luminescence (λmax ~ 540 nm); this corresponds to the emission of the Y-1 strain in vivo. This yellow bioluminescence emission is thus ascribed to the interaction of these two proteins, and to the excitation of the singlet FMN bound to this fluorescent protein.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690MACHEROUX, Peter, K. U. SCHMIDT, Petra STEINERSTAUCH, Sandro GHISLA, Pio COLEPICOLO, Rudolf BUNTIC, J. Woodland HASTINGS, 1987. Purification of the yellow fluorescent protein from vibrio fischeri and identity of the flavin chromophore. In: Biochemical and Biophysical Research Communications. 1987, 146(1), pp. 101-106. ISSN 0006-291X
BibTex
@article{Macheroux1987Purif-7105,
  year={1987},
  title={Purification of the yellow fluorescent protein from vibrio fischeri and identity of the flavin chromophore},
  number={1},
  volume={146},
  issn={0006-291X},
  journal={Biochemical and Biophysical Research Communications},
  pages={101--106},
  author={Macheroux, Peter and Schmidt, K. U. and Steinerstauch, Petra and Ghisla, Sandro and Colepicolo, Pio and Buntic, Rudolf and Hastings, J. Woodland}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7105">
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7105/1/Purification_of_the_yellow_fluorescent_protein_from_vibrio_fischeri_and_identity_of_the_flavin_chromophore.pdf"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:issued>1987</dcterms:issued>
    <dc:contributor>Buntic, Rudolf</dc:contributor>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:32Z</dcterms:available>
    <dc:contributor>Schmidt, K. U.</dc:contributor>
    <dcterms:bibliographicCitation>First publ. in: Biochemical and Biophysical Research Communications 146 (1987), 1, pp. 101-106</dcterms:bibliographicCitation>
    <dc:language>eng</dc:language>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7105/1/Purification_of_the_yellow_fluorescent_protein_from_vibrio_fischeri_and_identity_of_the_flavin_chromophore.pdf"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:31:32Z</dc:date>
    <dc:contributor>Steinerstauch, Petra</dc:contributor>
    <dc:format>application/pdf</dc:format>
    <dc:creator>Buntic, Rudolf</dc:creator>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7105"/>
    <dc:contributor>Colepicolo, Pio</dc:contributor>
    <dc:creator>Macheroux, Peter</dc:creator>
    <dc:contributor>Macheroux, Peter</dc:contributor>
    <dc:creator>Hastings, J. Woodland</dc:creator>
    <dc:creator>Ghisla, Sandro</dc:creator>
    <dc:creator>Colepicolo, Pio</dc:creator>
    <dc:creator>Schmidt, K. U.</dc:creator>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dc:creator>Steinerstauch, Petra</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Ghisla, Sandro</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:abstract xml:lang="eng">A low molecular weight protein (~ 25,000 D) exhibiting a yellow fluorescence emission peaking at ~ 540 nm was isolated from Vibrio fischeri (strain Y-1) and purified to apparent homogeneity. FMN is the chromophore, but it exhibits marked red shifts in both the absorption (λmax = 380, 460 nm) and the fluorescence emission. When added to purified luciferase from the same strain, which itself catalyzes an emission of blue-green light (λmax ~ 495 nm), this protein induces a bright yellow luminescence (λmax ~ 540 nm); this corresponds to the emission of the Y-1 strain in vivo. This yellow bioluminescence emission is thus ascribed to the interaction of these two proteins, and to the excitation of the singlet FMN bound to this fluorescent protein.</dcterms:abstract>
    <dcterms:title>Purification of the yellow fluorescent protein from vibrio fischeri and identity of the flavin chromophore</dcterms:title>
    <dc:contributor>Hastings, J. Woodland</dc:contributor>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen