Binding of the third Na+ ion to the cytoplasmatic side of the Na,K-ATPase is electrogenic

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1999
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Domaszewicz, Wolfgang
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Zusammenfassung

A new experimental setup was constructed to allow parallel measurements of total internal reflection fluorescence and of capacitance changes in Na,K-ATPase-containing membranes. Effects correlated with cytoplasmatic sodium binding to Na,K-ATPase were investigated. Ion binding-induced fluorescence changes of the electrochromic dye RH421 in membrane fragments adsorbed on a transparent capacitative electrode corresponded perfectly to capacitance increases detected by a lock-in technique. From these electric measurements it was possible to estimate a dielectric coefficient of about 0.25 for the electrogenetic binding of the third Na+ ion. Binding of K+ to cytoplasmic sites was electroneutral.

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570 Biowissenschaften, Biologie
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Sodium pump, Ion movement, Electrogenic binding, Fluorescent dye, Capacitative coupling
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ISO 690DOMASZEWICZ, Wolfgang, Hans-Jürgen APELL, 1999. Binding of the third Na+ ion to the cytoplasmatic side of the Na,K-ATPase is electrogenic. In: FEBS Letters. 1999, 458(2), pp. 241-246. ISSN 0014-5793. Available under: doi: 10.1016/S0014-5793(99)01162-X
BibTex
@article{Domaszewicz1999Bindi-7852,
  year={1999},
  doi={10.1016/S0014-5793(99)01162-X},
  title={Binding of the third Na+ ion to the cytoplasmatic side of the Na,K-ATPase is electrogenic},
  number={2},
  volume={458},
  issn={0014-5793},
  journal={FEBS Letters},
  pages={241--246},
  author={Domaszewicz, Wolfgang and Apell, Hans-Jürgen}
}
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