Quorum sensing by N-acylhomoserine lactones is not required for Aeromonas hydrophila during growth with organic particles in lake water microcosms

Abstract

It was investigated whether quorum sensing (QS) mediated by N-acylhomoserine lactones (AHLs) was important for heterotrophic bacteria from the littoral zone of the oligotrophic Lake Constance for growth with organic particles. More than 900 colonies from lake water microcosms with artificial organic aggregates consisting of autoclaved unicellular algae embedded in agarose beads were screened for AHL-production. AHL-producing bacteria of the genus Aeromonas enriched in the microcosms but AHLs could not be detected in any microcosm. To test for a potential function of AHL-mediated QS, growth experiments with the wild type and an AHL-deWcient mutant of Aeromonas hydrophila in lake water microcosms were performed. Growth of both strains did not diVer in single cultures and showed no mutual inXuence in co-cultures. In cocultures with a competitor bacterium belonging to the Cytophaga Flavobacterium group, growth of both A. hydrophila strains was reduced while growth of the competitor bacterium was not aVected. Exogenous AHL-addition did not inXuence growth of the Aeromonas strains in any microcosm experiment. These results showed that AHL-mediated QS was not required for A. hydrophila during colonization and degradation of organic particles in lake water microcosms, suggesting that cell cell signalling of heterotrophic bacteria in oligotrophic waters relies on novel signal molecules.