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Mode of interaction of the Gαo subunit of heterotrimeric G proteins with the GoLoco1 motif of Drosophila Pins is determined by guanine nucleotides

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2015

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Lüchtenborg, Anne-Marie
Melnik, Bogdan S.

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Bioscience Reports. 2015, 35(6), e00271. ISSN 0144-8463. eISSN 1573-4935. Available under: doi: 10.1042/BSR20150201

Zusammenfassung

Drosophila GoLoco motif-containing protein Pins is unusual in its highly efficient interaction with both GDP- and the GTP-loaded forms of the α-subunit of the heterotrimeric Go protein. We analysed the interactions of Gαo in its two nucleotide forms with GoLoco1-the first of the three GoLoco domains of Pins-and the possible structures of the resulting complexes, through combination of conventional fluorescence and FRET measurements as well as through molecular modelling. Our data suggest that the orientation of the GoLoco1 motif on Gαo significantly differs between the two nucleotide states of the latter. In other words, a rotation of the GoLoco1 peptide in respect with Gαo must accompany the nucleotide exchange in Gαo. The sterical hindrance requiring such a rotation probably contributes to the guanine nucleotide exchange inhibitor activity of GoLoco1 and Pins as a whole. Our data have important implications for the mechanisms of Pins regulation in the process of asymmetric cell divisions.

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570 Biowissenschaften, Biologie

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asymmetric cell division, FRET, GoLoco, heterotrimeric G proteins, nucleotide exchange

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ISO 690LÜCHTENBORG, Anne-Marie, Vladimir PURVANOV, Bogdan S. MELNIK, Simon BECKER, Vladimir L. KATANAEV, 2015. Mode of interaction of the Gαo subunit of heterotrimeric G proteins with the GoLoco1 motif of Drosophila Pins is determined by guanine nucleotides. In: Bioscience Reports. 2015, 35(6), e00271. ISSN 0144-8463. eISSN 1573-4935. Available under: doi: 10.1042/BSR20150201
BibTex
@article{Luchtenborg2015-12-01inter-40287,
  year={2015},
  doi={10.1042/BSR20150201},
  title={Mode of interaction of the Gαo subunit of heterotrimeric G proteins with the GoLoco1 motif of Drosophila Pins is determined by guanine nucleotides},
  number={6},
  volume={35},
  issn={0144-8463},
  journal={Bioscience Reports},
  author={Lüchtenborg, Anne-Marie and Purvanov, Vladimir and Melnik, Bogdan S. and Becker, Simon and Katanaev, Vladimir L.},
  note={Article Number: e00271}
}
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    <dcterms:abstract xml:lang="eng">Drosophila GoLoco motif-containing protein Pins is unusual in its highly efficient interaction with both GDP- and the GTP-loaded forms of the α-subunit of the heterotrimeric Go protein. We analysed the interactions of Gαo in its two nucleotide forms with GoLoco1-the first of the three GoLoco domains of Pins-and the possible structures of the resulting complexes, through combination of conventional fluorescence and FRET measurements as well as through molecular modelling. Our data suggest that the orientation of the GoLoco1 motif on Gαo significantly differs between the two nucleotide states of the latter. In other words, a rotation of the GoLoco1 peptide in respect with Gαo must accompany the nucleotide exchange in Gαo. The sterical hindrance requiring such a rotation probably contributes to the guanine nucleotide exchange inhibitor activity of GoLoco1 and Pins as a whole. Our data have important implications for the mechanisms of Pins regulation in the process of asymmetric cell divisions.</dcterms:abstract>
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