Chemoenzymatic Synthesis and Fluorescent Visualization of Cell-Surface Selectin-Bound Sialyl Lewis X Derivatives
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Chemistry - A European Journal. 2000, 6(1), pp. 162-171. ISSN 0947-6539. eISSN 1521-3765
Zusammenfassung
Sialyl Lewis x (sLex) derivatives conjugated to readily visualized molecular labels are useful chemical probes to study selectin-carbohydrate interactions. Localization of the selectins on the surface of leukocytes and activated endothelial cells can be detected through fluorescence of bound selectin ligands. Herein we present a short chemoenzymatic synthesis of a fluorescently labeled bivalent sLe× conjugate. The use of an amino-substituted monovalent sLe× to obtain fluorescent- and biotin-labeled sLe× derivatives is also described. The cell-staining utility of the fluorescent sLe× conjugates is demonstrated for a HUVEC cell line expressing E-selectin and for CHO-K1 cells expressing either L- or E-selectin.
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540 Chemie
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chemoenzymatic synthesis, oligosaccharides, regioselective glycosylation, selectin, sialyl Lewis x
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WITTMANN, Valentin, Arun K. DATTA, Kathryn M. KOELLER, Chi-Huey WONG, 2000. Chemoenzymatic Synthesis and Fluorescent Visualization of Cell-Surface Selectin-Bound Sialyl Lewis X Derivatives. In: Chemistry - A European Journal. 2000, 6(1), pp. 162-171. ISSN 0947-6539. eISSN 1521-3765BibTex
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year={2000},
title={Chemoenzymatic Synthesis and Fluorescent Visualization of Cell-Surface Selectin-Bound Sialyl Lewis X Derivatives},
number={1},
volume={6},
issn={0947-6539},
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pages={162--171},
author={Wittmann, Valentin and Datta, Arun K. and Koeller, Kathryn M. and Wong, Chi-Huey}
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