Chemoenzymatic Synthesis and Fluorescent Visualization of Cell-Surface Selectin-Bound Sialyl Lewis X Derivatives

Lade...
Vorschaubild
Dateien
Chemoenzymatic_Synthesis.pdf
Chemoenzymatic_Synthesis.pdfGröße: 277.34 KBDownloads: 601
Datum
2000
Autor:innen
Datta, Arun K.
Koeller, Kathryn M.
Wong, Chi-Huey
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
DOI (zitierfähiger Link)
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Sammlungen
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Zusammenfassung

Sialyl Lewis x (sLex) derivatives conjugated to readily visualized molecular labels are useful chemical probes to study selectin-carbohydrate interactions. Localization of the selectins on the surface of leukocytes and activated endothelial cells can be detected through fluorescence of bound selectin ligands. Herein we present a short chemoenzymatic synthesis of a fluorescently labeled bivalent sLe× conjugate. The use of an amino-substituted monovalent sLe× to obtain fluorescent- and biotin-labeled sLe× derivatives is also described. The cell-staining utility of the fluorescent sLe× conjugates is demonstrated for a HUVEC cell line expressing E-selectin and for CHO-K1 cells expressing either L- or E-selectin.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
Schlagwörter
chemoenzymatic synthesis, oligosaccharides, regioselective glycosylation, selectin, sialyl Lewis x
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690WITTMANN, Valentin, Arun K. DATTA, Kathryn M. KOELLER, Chi-Huey WONG, 2000. Chemoenzymatic Synthesis and Fluorescent Visualization of Cell-Surface Selectin-Bound Sialyl Lewis X Derivatives. In: Chemistry - A European Journal. 2000, 6(1), pp. 162-171. ISSN 0947-6539. eISSN 1521-3765
BibTex
@article{Wittmann2000Chemo-9811,
  year={2000},
  title={Chemoenzymatic Synthesis and Fluorescent Visualization of Cell-Surface Selectin-Bound Sialyl Lewis X Derivatives},
  number={1},
  volume={6},
  issn={0947-6539},
  journal={Chemistry - A European Journal},
  pages={162--171},
  author={Wittmann, Valentin and Datta, Arun K. and Koeller, Kathryn M. and Wong, Chi-Huey}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/9811">
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/9811/1/Chemoenzymatic_Synthesis.pdf"/>
    <dcterms:issued>2000</dcterms:issued>
    <dc:format>application/pdf</dc:format>
    <dc:creator>Koeller, Kathryn M.</dc:creator>
    <dcterms:title>Chemoenzymatic Synthesis and Fluorescent Visualization of Cell-Surface Selectin-Bound Sialyl Lewis X Derivatives</dcterms:title>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dcterms:abstract xml:lang="eng">Sialyl Lewis x (sLex) derivatives conjugated to readily visualized molecular labels are useful chemical probes to study selectin-carbohydrate interactions. Localization of the selectins on the surface of leukocytes and activated endothelial cells can be detected through fluorescence of bound selectin ligands. Herein we present a short chemoenzymatic synthesis of a fluorescently labeled bivalent sLe× conjugate. The use of an amino-substituted monovalent sLe× to obtain fluorescent- and biotin-labeled sLe× derivatives is also described. The cell-staining utility of the fluorescent sLe× conjugates is demonstrated for a HUVEC cell line expressing E-selectin and for CHO-K1 cells expressing either L- or E-selectin.</dcterms:abstract>
    <dc:creator>Wittmann, Valentin</dc:creator>
    <dcterms:bibliographicCitation>First publ. in: Chemistry - A European Journal 6 (2000), 1, pp. 162-171</dcterms:bibliographicCitation>
    <dc:creator>Wong, Chi-Huey</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:14:34Z</dc:date>
    <dc:contributor>Wong, Chi-Huey</dc:contributor>
    <dc:contributor>Wittmann, Valentin</dc:contributor>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/9811"/>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:language>eng</dc:language>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/9811/1/Chemoenzymatic_Synthesis.pdf"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:14:34Z</dcterms:available>
    <dc:contributor>Datta, Arun K.</dc:contributor>
    <dc:creator>Datta, Arun K.</dc:creator>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Koeller, Kathryn M.</dc:contributor>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet
Diese Publikation teilen