Chemoenzymatic Synthesis and Fluorescent Visualization of Cell-Surface Selectin-Bound Sialyl Lewis X Derivatives
Chemoenzymatic Synthesis and Fluorescent Visualization of Cell-Surface Selectin-Bound Sialyl Lewis X Derivatives
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2000
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Chemistry - A European Journal ; 6 (2000), 1. - pp. 162-171. - ISSN 0947-6539. - eISSN 1521-3765
Abstract
Sialyl Lewis x (sLex) derivatives conjugated to readily visualized molecular labels are useful chemical probes to study selectin-carbohydrate interactions. Localization of the selectins on the surface of leukocytes and activated endothelial cells can be detected through fluorescence of bound selectin ligands. Herein we present a short chemoenzymatic synthesis of a fluorescently labeled bivalent sLe× conjugate. The use of an amino-substituted monovalent sLe× to obtain fluorescent- and biotin-labeled sLe× derivatives is also described. The cell-staining utility of the fluorescent sLe× conjugates is demonstrated for a HUVEC cell line expressing E-selectin and for CHO-K1 cells expressing either L- or E-selectin.
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540 Chemistry
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chemoenzymatic synthesis,oligosaccharides,regioselective glycosylation,selectin,sialyl Lewis x
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WITTMANN, Valentin, Arun K. DATTA, Kathryn M. KOELLER, Chi-Huey WONG, 2000. Chemoenzymatic Synthesis and Fluorescent Visualization of Cell-Surface Selectin-Bound Sialyl Lewis X Derivatives. In: Chemistry - A European Journal. 6(1), pp. 162-171. ISSN 0947-6539. eISSN 1521-3765BibTex
@article{Wittmann2000Chemo-9811, year={2000}, title={Chemoenzymatic Synthesis and Fluorescent Visualization of Cell-Surface Selectin-Bound Sialyl Lewis X Derivatives}, number={1}, volume={6}, issn={0947-6539}, journal={Chemistry - A European Journal}, pages={162--171}, author={Wittmann, Valentin and Datta, Arun K. and Koeller, Kathryn M. and Wong, Chi-Huey} }
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