@article{Wittmann2000Chemo-9811,
  year={2000},
  title={Chemoenzymatic Synthesis and Fluorescent Visualization of Cell-Surface Selectin-Bound Sialyl Lewis X Derivatives},
  number={1},
  volume={6},
  issn={0947-6539},
  journal={Chemistry - A European Journal},
  pages={162--171},
  author={Wittmann, Valentin and Datta, Arun K. and Koeller, Kathryn M. and Wong, Chi-Huey}
}

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    <dcterms:title>Chemoenzymatic Synthesis and Fluorescent Visualization of Cell-Surface Selectin-Bound Sialyl Lewis X Derivatives</dcterms:title>
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    <dcterms:abstract xml:lang="eng">Sialyl Lewis x (sLex) derivatives conjugated to readily visualized molecular labels are useful chemical probes to study selectin-carbohydrate interactions. Localization of the selectins on the surface of leukocytes and activated endothelial cells can be detected through fluorescence of bound selectin ligands. Herein we present a short chemoenzymatic synthesis of a fluorescently labeled bivalent sLe× conjugate. The use of an amino-substituted monovalent sLe× to obtain fluorescent- and biotin-labeled sLe× derivatives is also described. The cell-staining utility of the fluorescent sLe× conjugates is demonstrated for a HUVEC cell line expressing E-selectin and for CHO-K1 cells expressing either L- or E-selectin.</dcterms:abstract>
    <dc:creator>Wittmann, Valentin</dc:creator>
    <dcterms:bibliographicCitation>First publ. in: Chemistry - A European Journal 6 (2000), 1, pp. 162-171</dcterms:bibliographicCitation>
    <dc:creator>Wong, Chi-Huey</dc:creator>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:14:34Z</dc:date>
    <dc:contributor>Wong, Chi-Huey</dc:contributor>
    <dc:contributor>Wittmann, Valentin</dc:contributor>
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    <dc:contributor>Datta, Arun K.</dc:contributor>
    <dc:creator>Datta, Arun K.</dc:creator>
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