Functional and fluorochrome analysis of an exocytotic mutant yields evidence of store-operated Ca2+ influx in Paramecium

Lade...
Vorschaubild
Dateien
Functional_and_fluorochrome_analysis.pdf
Functional_and_fluorochrome_analysis.pdfGröße: 437.59 KBDownloads: 252
Datum
2002
Autor:innen
Mohamed, Ihab Kamal
Klauke, Norbert
Cohen, Jean
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
EU-Projektnummer
DFG-Projektnummer
Projekt
TP C4: Microdomain calcium signalling in a secretory protozoan cell: Identification and functional localization of membrane proteins in cortical calcium stores
Funktionell-ultrastrukturelle Analyse des Exocytose-Prozesses (Sekretausschleusung)
Open Access-Veröffentlichung
Sammlungen
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
unikn.publication.listelement.citation.prefix.version.undefined
Journal of Membrane Biology. 2002, 187(1), pp. 1-14. ISSN 0022-2631. eISSN 1432-1424. Available under: doi: 10.1007/s00232-001-0146-6
Zusammenfassung

A non-discharge mutant of Paramecium tetraurelia (nd12-35°C, lacking exocytotic response upon stimulation with the nonpermeable polycationic secretagogue aminoethyldextran, AED), in the pawnA genetic context (d4-500r, lacking ciliary voltage-dependent Ca2+ influx), was shown to lack 45Ca2+ entry from outside upon AED stimulation. In contrast, cells grown at 25°C behave like the wildtype. To check the functional properties in more detail, fluorochrome-loaded 35°C cells were stimulated, not only with AED (EC100 = 10-6 M in wildtype cells), but also with 4-chloro-meta-cresol, (4CmC, 0.5 mM), a permeable activator of ryanodine receptor-type Ca2+ release channels, usually at extracellular [Ca2+] of 50 mM, and eventually with a Ca2+ chelator added. We confirm that pwA-nd12(35°C) cells lack any Ca2+ influx and any exocytosis of trichocysts in response to any stimulus. As we determined by x-ray microanalysis, total calcium content in alveolar sacs (subplasmalemmal stores) known to be mobilized upon exocytosis stimulation in wild-type cells, contain about the same total calcium in 35°C as in 25°C cells, and Ca2+ mobilization from alveoli by AED or 4CmC is also nearly the same. Due to the absence of any AED-induced Ca2+ influx in 35°C cells and normal Ca2+ release from stores found by x-ray microanalysis one can exclude a "CICR"-type mechanism (Ca2+-induced Ca2+ release) and imply that normally a store-operated Ca2+ ("SOC") influx would occur (as in 25°C cells). Furthermore, 35°C cells display a significantly lower basal intracellular [Ca2+], so that any increase upon stimulation may be less expressed or even remain undetected. Under these conditions, any mobilization of Ca2+ from stores cannot compensate for the lack of Ca2+ influx, particularly since normally both components have to cooperate to achieve full exocytotic response. Also striking is our finding that 35°C cells are unable to perform membrane fusion, as analyzed with the Ca2+ ionophore, A23187. These findings were corroborated by cryofixation and freeze-fracture analysis of trichocyst docking sites after AED or 4CmC stimulation, which also revealed no membrane fusion. In sum, in nd12 cells increased culture temperature entails multiple defects, notably insensitivity to any Ca2+ signal, which, moreover, cannot develop properly due to a lower basal [Ca2+] level and the lack of Ca2+ influx, despite normal store activation.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
570 Biowissenschaften, Biologie
Schlagwörter
Ca2+, Calcium, Exocytosis, Paramecium, Secretion
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690MOHAMED, Ihab Kamal, Norbert KLAUKE, Joachim HENTSCHEL, Jean COHEN, Helmut PLATTNER, 2002. Functional and fluorochrome analysis of an exocytotic mutant yields evidence of store-operated Ca2+ influx in Paramecium. In: Journal of Membrane Biology. 2002, 187(1), pp. 1-14. ISSN 0022-2631. eISSN 1432-1424. Available under: doi: 10.1007/s00232-001-0146-6
BibTex
@article{Mohamed2002Funct-8651,
  year={2002},
  doi={10.1007/s00232-001-0146-6},
  title={Functional and fluorochrome analysis of an exocytotic mutant yields evidence of store-operated Ca2+ influx in Paramecium},
  number={1},
  volume={187},
  issn={0022-2631},
  journal={Journal of Membrane Biology},
  pages={1--14},
  author={Mohamed, Ihab Kamal and Klauke, Norbert and Hentschel, Joachim and Cohen, Jean and Plattner, Helmut}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8651">
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8651"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8651/1/Functional_and_fluorochrome_analysis.pdf"/>
    <dcterms:title>Functional and fluorochrome analysis of an exocytotic mutant yields evidence of store-operated Ca2+ influx in Paramecium</dcterms:title>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <dc:creator>Mohamed, Ihab Kamal</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Cohen, Jean</dc:creator>
    <dc:contributor>Cohen, Jean</dc:contributor>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Hentschel, Joachim</dc:creator>
    <dc:contributor>Hentschel, Joachim</dc:contributor>
    <dc:creator>Plattner, Helmut</dc:creator>
    <dc:format>application/pdf</dc:format>
    <dcterms:abstract xml:lang="eng">A non-discharge mutant of Paramecium tetraurelia (nd12-35°C, lacking exocytotic response upon stimulation with the nonpermeable polycationic secretagogue aminoethyldextran, AED), in the pawnA genetic context (d4-500r, lacking ciliary voltage-dependent Ca2+ influx), was shown to lack 45Ca2+ entry from outside upon AED stimulation. In contrast, cells grown at 25°C behave like the wildtype. To check the functional properties in more detail, fluorochrome-loaded 35°C cells were stimulated, not only with AED (EC100 = 10-6 M in wildtype cells), but also with 4-chloro-meta-cresol, (4CmC, 0.5 mM), a permeable activator of ryanodine receptor-type Ca2+ release channels, usually at extracellular [Ca2+] of 50 mM, and eventually with a Ca2+ chelator added. We confirm that pwA-nd12(35°C) cells lack any Ca2+ influx and any exocytosis of trichocysts in response to any stimulus. As we determined by x-ray microanalysis, total calcium content in alveolar sacs (subplasmalemmal stores) known to be mobilized upon exocytosis stimulation in wild-type cells, contain about the same total calcium in 35°C as in 25°C cells, and Ca2+ mobilization from alveoli by AED or 4CmC is also nearly the same. Due to the absence of any AED-induced Ca2+ influx in 35°C cells and normal Ca2+ release from stores found by x-ray microanalysis one can exclude a "CICR"-type mechanism (Ca2+-induced Ca2+ release) and imply that normally a store-operated Ca2+ ("SOC") influx would occur (as in 25°C cells). Furthermore, 35°C cells display a significantly lower basal intracellular [Ca2+], so that any increase upon stimulation may be less expressed or even remain undetected. Under these conditions, any mobilization of Ca2+ from stores cannot compensate for the lack of Ca2+ influx, particularly since normally both components have to cooperate to achieve full exocytotic response. Also striking is our finding that 35°C cells are unable to perform membrane fusion, as analyzed with the Ca2+ ionophore, A23187. These findings were corroborated by cryofixation and freeze-fracture analysis of trichocyst docking sites after AED or 4CmC stimulation, which also revealed no membrane fusion. In sum, in nd12 cells increased culture temperature entails multiple defects, notably insensitivity to any Ca2+ signal, which, moreover, cannot develop properly due to a lower basal [Ca2+] level and the lack of Ca2+ influx, despite normal store activation.</dcterms:abstract>
    <dc:contributor>Plattner, Helmut</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dcterms:bibliographicCitation>First publ. in: Journal of Membrane Biology ; 187 (2002). - S. 1-14</dcterms:bibliographicCitation>
    <dc:contributor>Klauke, Norbert</dc:contributor>
    <dc:creator>Klauke, Norbert</dc:creator>
    <dc:language>eng</dc:language>
    <dcterms:issued>2002</dcterms:issued>
    <dc:contributor>Mohamed, Ihab Kamal</dc:contributor>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8651/1/Functional_and_fluorochrome_analysis.pdf"/>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:45:24Z</dcterms:available>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:45:24Z</dc:date>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Nein
Begutachtet