Siderophore-Mediated Iron Transport : Crystal Structure of FhuA with Bound Lipopolysaccharide

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1998
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Ferguson, Andrew D.
Hofmann, Eckhard
Coulton, James W.
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Science. 1998, 282(5397), pp. 2215-2220. Available under: doi: 10.1126/science.282.5397.2215
Zusammenfassung

FhuA, the receptor for ferrichrome-iron in Escherichia coli, is a member of a family of integral outer membrane proteins, which, together with the energytransducing protein TonB, mediate the active transport of ferric siderophores across the outer membrane of Gram-negative bacteria. The three-dimensional structure of FhuA is presented here in two conformations: with and without ferrichrome-iron at resolutions of 2.7 and 2.5 angstroms, respectively. FhuA is a β barrel composed of 22 antiparallel β strands. In contrast to the typical trimeric arrangement found in porins, FhuA is monomeric. Located within the β barrel is a structurally distinct domain, the "cork," which mainly consists of a four-stranded β sheet and four short α helices. A single lipopolysaccharide molecule is noncovalently associated with the membrane-embedded region of the protein. Upon binding of ferrichrome-iron, conformational changes are transduced to the periplasmic pocket of FhuA, signaling the ligand-loaded status of the receptor. Sequence homologies and mutagenesis data are used to propose a structural mechanism for TonB-dependent siderophore-mediated transport across the outer membrane.

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570 Biowissenschaften, Biologie
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ISO 690FERGUSON, Andrew D., Eckhard HOFMANN, James W. COULTON, Kay DIEDERICHS, Wolfram WELTE, 1998. Siderophore-Mediated Iron Transport : Crystal Structure of FhuA with Bound Lipopolysaccharide. In: Science. 1998, 282(5397), pp. 2215-2220. Available under: doi: 10.1126/science.282.5397.2215
BibTex
@article{Ferguson1998Sider-8631,
  year={1998},
  doi={10.1126/science.282.5397.2215},
  title={Siderophore-Mediated Iron Transport : Crystal Structure of FhuA with Bound Lipopolysaccharide},
  number={5397},
  volume={282},
  journal={Science},
  pages={2215--2220},
  author={Ferguson, Andrew D. and Hofmann, Eckhard and Coulton, James W. and Diederichs, Kay and Welte, Wolfram}
}
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