Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts

Tournebize, Régis; Popov, Andrei; Kinoshita, Kazuhisa; Ashford, Anthony J.; Rybina, Sonja; Pozniakovsky, Andrei; Mayer, Thomas U.; Walczak, Claire E.; Karsenti, Eric; Hyman, Anthony A.

Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts

Dateien

Tournebize_140552.pdfGröße: 796.91 KBDownloads: 621

Datum

2000

Autor:innen

Tournebize, Régis

Popov, Andrei

Kinoshita, Kazuhisa

Ashford, Anthony J.

Rybina, Sonja

Pozniakovsky, Andrei

Mayer, Thomas U.

Walczak, Claire E.

Karsenti, Eric

Hyman, Anthony A.

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Nature Cell Biology. 2000, 2(1), pp. 13-19. ISSN 1465-7392. Available under: doi: 10.1038/71330

Zusammenfassung

Microtubules are dynamic polymers that move stochastically between periods of growth and shrinkage, a property known as dynamic instability. Here, to investigate the mechanisms regulating microtubule dynamics in Xenopus egg extracts, we have cloned the complementary DNA encoding the microtubule-associated protein XMAP215 and investigated the function of the XMAP215 protein. Immunodepletion of XMAP215 indicated that it is a major microtubule-stabilizing factor in Xenopus egg extracts. During interphase, XMAP215 stabilizes microtubules primarily by opposing the activity of the destabilizing factor XKCM1, a member of the kinesin superfamily. These results indicate that microtubule dynamics in Xenopus egg extracts are regulated by a balance between a stabilizing factor, XMAP215, and a destabilizing factor, XKCM1.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Schlagwörter

MCAK, XMAP215, microtubule dynamics, Mitose, Chromosomenbande, Zellteilung, Mikrotubuli, Kinesin

Zitieren

ISO 690

TOURNEBIZE, Régis, Andrei POPOV, Kazuhisa KINOSHITA, Anthony J. ASHFORD, Sonja RYBINA, Andrei POZNIAKOVSKY, Thomas U. MAYER, Claire E. WALCZAK, Eric KARSENTI, Anthony A. HYMAN, 2000. Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts. In: Nature Cell Biology. 2000, 2(1), pp. 13-19. ISSN 1465-7392. Available under: doi: 10.1038/71330

BibTex

@article{Tournebize2000-01Contr-14055,
  year={2000},
  doi={10.1038/71330},
  title={Control of microtubule dynamics by the antagonistic activities of XMAP215 and XKCM1 in Xenopus egg extracts},
  number={1},
  volume={2},
  issn={1465-7392},
  journal={Nature Cell Biology},
  pages={13--19},
  author={Tournebize, Régis and Popov, Andrei and Kinoshita, Kazuhisa and Ashford, Anthony J. and Rybina, Sonja and Pozniakovsky, Andrei and Mayer, Thomas U. and Walczak, Claire E. and Karsenti, Eric and Hyman, Anthony A.}
}

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Universitätsbibliographie

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