CR-LAAO antileukemic effect against Bcr-Abl+ cells is mediated by apoptosis and hydrogen peroxide

No Thumbnail Available
Files
There are no files associated with this item.
Date
2016
Authors
Burin, Sandra Mara
Ouchida, Amanda Tomie
Aissa, Alexandre Ferro
Coelho, Maria Gabriela Berzoti
Costa, Tássia Rafaella
Marsola, Ana Paula Zambuzi Cardoso
Pinto-Simões, Belinda
Antunes, Lusânia Maria Greggi
de Castro, Fabíola Attié
Editors
Contact
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
URI (citable link)
ArXiv-ID
International patent number
Link to the license
oops
EU project number
Project
Open Access publication
Collections
Restricted until
Title in another language
Research Projects
Organizational Units
Journal Issue
Publication type
Journal article
Publication status
Published
Published in
International Journal of Biological Macromolecules ; 86 (2016). - pp. 309-320. - ISSN 0141-8130. - eISSN 1879-0003
Abstract
Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm characterized by the presence of the Bcr-Abl tyrosine kinase protein, which confers resistance to apoptosis in leukemic cells. Tyrosine kinase inhibitors (TKIs) are effectively used to treat CML; however, CML patients in the advanced (CML-AP) and chronic (CML-CP) phases of the disease are usually resistant to TKI therapy. Thus, it is necessary to seek for novel agents to treat CML, such as the enzyme l-amino acid oxidase from Calloselasma rhodostoma (CR-LAAO) snake venom. We examined the antitumor effect of CR-LAAO in Bcr-Abl(+) cell lines and peripheral blood mononuclear cells (PBMC) from healthy subjects and CML patients. CR-LAAO was more cytotoxic towards Bcr-Abl(+) cell lines than towards healthy subjects' PBMC. The H2O2 produced during the enzymatic action of CR-LAAO mediated its cytotoxic effect. The CR-LAAO induced apoptosis in Bcr-Abl(+) cells, as detected by caspases 3, 8, and 9 activation, loss of mitochondrial membrane potential, and DNA damage. CR-LAAO elicited apoptosis in PBMC from CML-CP patients without TKI treatment more strongly than in PBMC from healthy subjects and TKI-treated CML-CP and CML-AP patients. The antitumor effect of CR-LAAO against Bcr-Abl(+) cells makes this toxin a promising candidate to CML therapy.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690BURIN, Sandra Mara, Sandro GHISLA, Amanda Tomie OUCHIDA, Alexandre Ferro AISSA, Maria Gabriela Berzoti COELHO, Tássia Rafaella COSTA, Ana Paula Zambuzi Cardoso MARSOLA, Belinda PINTO-SIMÕES, Lusânia Maria Greggi ANTUNES, Fabíola Attié DE CASTRO, 2016. CR-LAAO antileukemic effect against Bcr-Abl+ cells is mediated by apoptosis and hydrogen peroxide. In: International Journal of Biological Macromolecules. 86, pp. 309-320. ISSN 0141-8130. eISSN 1879-0003. Available under: doi: 10.1016/j.ijbiomac.2016.01.069
BibTex
@article{Burin2016CRLAA-34275,
  year={2016},
  doi={10.1016/j.ijbiomac.2016.01.069},
  title={CR-LAAO antileukemic effect against Bcr-Abl+ cells is mediated by apoptosis and hydrogen peroxide},
  volume={86},
  issn={0141-8130},
  journal={International Journal of Biological Macromolecules},
  pages={309--320},
  author={Burin, Sandra Mara and Ghisla, Sandro and Ouchida, Amanda Tomie and Aissa, Alexandre Ferro and Coelho, Maria Gabriela Berzoti and Costa, Tássia Rafaella and Marsola, Ana Paula Zambuzi Cardoso and Pinto-Simões, Belinda and Antunes, Lusânia Maria Greggi and de Castro, Fabíola Attié}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/34275">
    <dc:contributor>Coelho, Maria Gabriela Berzoti</dc:contributor>
    <dc:contributor>Pinto-Simões, Belinda</dc:contributor>
    <dc:contributor>Marsola, Ana Paula Zambuzi Cardoso</dc:contributor>
    <dc:creator>Costa, Tássia Rafaella</dc:creator>
    <dc:contributor>de Castro, Fabíola Attié</dc:contributor>
    <dc:creator>Aissa, Alexandre Ferro</dc:creator>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-06-08T08:35:16Z</dcterms:available>
    <dc:contributor>Costa, Tássia Rafaella</dc:contributor>
    <dc:contributor>Antunes, Lusânia Maria Greggi</dc:contributor>
    <dc:contributor>Burin, Sandra Mara</dc:contributor>
    <dc:creator>Burin, Sandra Mara</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Marsola, Ana Paula Zambuzi Cardoso</dc:creator>
    <dc:language>eng</dc:language>
    <dc:creator>Coelho, Maria Gabriela Berzoti</dc:creator>
    <dc:contributor>Ouchida, Amanda Tomie</dc:contributor>
    <dc:creator>Antunes, Lusânia Maria Greggi</dc:creator>
    <dc:creator>Ghisla, Sandro</dc:creator>
    <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/34275"/>
    <dc:contributor>Aissa, Alexandre Ferro</dc:contributor>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>de Castro, Fabíola Attié</dc:creator>
    <dc:contributor>Ghisla, Sandro</dc:contributor>
    <dcterms:issued>2016</dcterms:issued>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2016-06-08T08:35:16Z</dc:date>
    <dc:creator>Ouchida, Amanda Tomie</dc:creator>
    <dc:creator>Pinto-Simões, Belinda</dc:creator>
    <dcterms:title>CR-LAAO antileukemic effect against Bcr-Abl+ cells is mediated by apoptosis and hydrogen peroxide</dcterms:title>
    <dcterms:abstract xml:lang="eng">Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm characterized by the presence of the Bcr-Abl tyrosine kinase protein, which confers resistance to apoptosis in leukemic cells. Tyrosine kinase inhibitors (TKIs) are effectively used to treat CML; however, CML patients in the advanced (CML-AP) and chronic (CML-CP) phases of the disease are usually resistant to TKI therapy. Thus, it is necessary to seek for novel agents to treat CML, such as the enzyme l-amino acid oxidase from Calloselasma rhodostoma (CR-LAAO) snake venom. We examined the antitumor effect of CR-LAAO in Bcr-Abl(+) cell lines and peripheral blood mononuclear cells (PBMC) from healthy subjects and CML patients. CR-LAAO was more cytotoxic towards Bcr-Abl(+) cell lines than towards healthy subjects' PBMC. The H2O2 produced during the enzymatic action of CR-LAAO mediated its cytotoxic effect. The CR-LAAO induced apoptosis in Bcr-Abl(+) cells, as detected by caspases 3, 8, and 9 activation, loss of mitochondrial membrane potential, and DNA damage. CR-LAAO elicited apoptosis in PBMC from CML-CP patients without TKI treatment more strongly than in PBMC from healthy subjects and TKI-treated CML-CP and CML-AP patients. The antitumor effect of CR-LAAO against Bcr-Abl(+) cells makes this toxin a promising candidate to CML therapy.</dcterms:abstract>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
  </rdf:Description>
</rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Contact
URL of original publication
Test date of URL
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
Yes
Refereed