Fermentative degradation of glycolic acid by defined syntrophic cocultures

Loading...
Thumbnail Image
Date
1991
Authors
Friedrich, Michael
Laderer, Ute
Editors
Contact
Journal ISSN
Electronic ISSN
ISBN
Bibliographical data
Publisher
Series
DOI (citable link)
ArXiv-ID
International patent number
EU project number
Project
Open Access publication
Collections
Restricted until
Title in another language
Research Projects
Organizational Units
Journal Issue
Publication type
Journal article
Publication status
Published in
Archives of Microbiology ; 156 (1991), 5. - pp. 398-404. - ISSN 0302-8933. - eISSN 1432-072X
Abstract
Three different defined cocultures of glycolatedegrading strictly anaerobic bacteria were isolated from enrichment cultures inoculated with freshwater sediment samples. Each culture contained a primary fermenting bacterium which used only glycolate as growth substrate. These cells were gram-positive, formed terminal oval spores, and did not contain cytochromes. Growth with glycolate was possible only in coculture with either a homoacetogenic bacterium or a hydrogen-utilizing methanogenic bacterium; the overall fermentation balance was either 4 glycolate ~3 acetate + 2CO2, or 4 glycolate ~ 3 CH4 + 5 CO2. These transformations indicate that glycolate was converted by the primary fermenting bacterium entirely to CO2 and reducing equivalents which were transferred to the partner organisms, probably through interspecies hydrogen transfer. The key enzymes of fermentative glycolate degradation were identified in cell-free extracts. An acetyl-CoA and ADP-dependent glyoxylate-converting enzyme activity, malic enzyme, pyruvate synthase, and methyl viologendependent hydrogenase were found at comparably high activities suggesting that these bacteria oxidize lycolate through a new pathway via malyl-CoA, and that ATP is synthesized by substrate-level phosphorylation, in a similar manner as found in a recently isolated glyoxylatefermenting anaerobe.
Summary in another language
Subject (DDC)
570 Biosciences, Biology
Keywords
Interspecies hydrogen transfer,Methanogenesis,Homoacetogenesis,Malic enzyme,Substrate level phosphorylation
Conference
Review
undefined / . - undefined, undefined. - (undefined; undefined)
Cite This
ISO 690FRIEDRICH, Michael, Ute LADERER, Bernhard SCHINK, 1991. Fermentative degradation of glycolic acid by defined syntrophic cocultures. In: Archives of Microbiology. 156(5), pp. 398-404. ISSN 0302-8933. eISSN 1432-072X. Available under: doi: 10.1007/BF00248717
BibTex
@article{Friedrich1991Ferme-7318,
  year={1991},
  doi={10.1007/BF00248717},
  title={Fermentative degradation of glycolic acid by defined syntrophic cocultures},
  number={5},
  volume={156},
  issn={0302-8933},
  journal={Archives of Microbiology},
  pages={398--404},
  author={Friedrich, Michael and Laderer, Ute and Schink, Bernhard}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/7318">
    <dc:contributor>Laderer, Ute</dc:contributor>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7318"/>
    <dcterms:abstract xml:lang="eng">Three different defined cocultures of glycolatedegrading strictly anaerobic bacteria were isolated from enrichment cultures inoculated with freshwater sediment samples. Each culture contained a primary fermenting bacterium which used only glycolate as growth substrate. These cells were gram-positive, formed terminal oval spores, and did not contain cytochromes. Growth with glycolate was possible only in coculture with either a homoacetogenic bacterium or a hydrogen-utilizing methanogenic bacterium; the overall fermentation balance was either 4 glycolate ~3 acetate + 2CO2, or 4 glycolate ~ 3 CH4 + 5 CO2. These transformations indicate that glycolate was converted by the primary fermenting bacterium entirely to CO2 and reducing equivalents which were transferred to the partner organisms, probably through interspecies hydrogen transfer. The key enzymes of fermentative glycolate degradation were identified in cell-free extracts. An acetyl-CoA and ADP-dependent glyoxylate-converting enzyme activity, malic enzyme, pyruvate synthase, and methyl viologendependent hydrogenase were found at comparably high activities suggesting that these bacteria oxidize lycolate through a new pathway via malyl-CoA, and that ATP is synthesized by substrate-level phosphorylation, in a similar manner as found in a recently isolated glyoxylatefermenting anaerobe.</dcterms:abstract>
    <dc:creator>Laderer, Ute</dc:creator>
    <dcterms:rights rdf:resource="http://creativecommons.org/licenses/by-nc-nd/2.0/"/>
    <dcterms:issued>1991</dcterms:issued>
    <dc:creator>Schink, Bernhard</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:language>eng</dc:language>
    <dc:rights>Attribution-NonCommercial-NoDerivs 2.0 Generic</dc:rights>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Friedrich, Michael</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7318/1/Fermentative_degradation_of_glycolic_acid_1991.pdf"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dc:contributor>Schink, Bernhard</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:contributor>Friedrich, Michael</dc:contributor>
    <dc:format>application/pdf</dc:format>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7318/1/Fermentative_degradation_of_glycolic_acid_1991.pdf"/>
    <dcterms:title>Fermentative degradation of glycolic acid by defined syntrophic cocultures</dcterms:title>
    <dcterms:bibliographicCitation>First publ. in: Archives of Microbiology 156 (1991), 5, pp. 398-404</dcterms:bibliographicCitation>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:33:29Z</dc:date>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:33:29Z</dcterms:available>
  </rdf:Description>
</rdf:RDF>
Internal note
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Contact
URL of original publication
Test date of URL
Examination date of dissertation
Method of financing
Comment on publication
Alliance license
Corresponding Authors der Uni Konstanz vorhanden
International Co-Authors
Bibliography of Konstanz
No
Refereed