Structures of DNA Polymerases caught processing size-augmented nucleotide probes

Lade...
Vorschaubild
Dateien
Betz_Angew_Chem_Int_Ed_2010.pdf
Betz_Angew_Chem_Int_Ed_2010.pdfGröße: 607.04 KBDownloads: 1224
Datum
2010
Herausgeber:innen
Kontakt
ISSN der Zeitschrift
Electronic ISSN
ISBN
Bibliografische Daten
Verlag
Schriftenreihe
Auflagebezeichnung
ArXiv-ID
Internationale Patentnummer
Angaben zur Forschungsförderung
Projekt
Open Access-Veröffentlichung
Open Access Green
Core Facility der Universität Konstanz
Gesperrt bis
Titel in einer weiteren Sprache
Forschungsvorhaben
Organisationseinheiten
Zeitschriftenheft
Publikationstyp
Zeitschriftenartikel
Publikationsstatus
Published
Erschienen in
Angewandte Chemie : International Edition. 2010, 49(30), pp. 5181-5184. eISSN 1521-3773. Available under: doi: 10.1002/anie.200905724
Zusammenfassung

The integrity of the genome relies primarily on the ability of DNA polymerases to efficiently catalyze selective DNA synthesis according to the Watson Crick rule in a templatedirected manner during DNA replication, repair, and recombination. Remarkably, some DNA polymerases achieve selective information transfer to the offspring in line with the Watson Crick rule with intrinsic error rates as low as one mistake per one million synthesized nucleotides.[1] This is far below the value that would be expected based on the energetic differences between canonical (i.e. Watson Crick) and noncanonical nucleobase pairing.[1] Geometric factors are widely cited to govern DNA polymerase selectivity.[1] Thus, high-fidelity DNA polymerases are believed to mostly select the canonical nucleotide based on close steric complementarity of the nascent base pair to the geometry of the active site of the enzyme.

Zusammenfassung in einer weiteren Sprache
Fachgebiet (DDC)
540 Chemie
Schlagwörter
Konferenz
Rezension
undefined / . - undefined, undefined
Zitieren
ISO 690BETZ, Karin, Frank STRECKENBACH, Andreas SCHNUR, Thomas E. EXNER, Wolfram WELTE, Kay DIEDERICHS, Andreas MARX, 2010. Structures of DNA Polymerases caught processing size-augmented nucleotide probes. In: Angewandte Chemie : International Edition. 2010, 49(30), pp. 5181-5184. eISSN 1521-3773. Available under: doi: 10.1002/anie.200905724
BibTex
@article{Betz2010Struc-9636,
  year={2010},
  doi={10.1002/anie.200905724},
  title={Structures of DNA Polymerases caught processing size-augmented nucleotide probes},
  number={30},
  volume={49},
  journal={Angewandte Chemie : International Edition},
  pages={5181--5184},
  author={Betz, Karin and Streckenbach, Frank and Schnur, Andreas and Exner, Thomas E. and Welte, Wolfram and Diederichs, Kay and Marx, Andreas}
}
RDF
<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/9636">
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/9636/1/Betz_Angew_Chem_Int_Ed_2010.pdf"/>
    <dcterms:abstract xml:lang="eng">The integrity of the genome relies primarily on the ability of DNA polymerases to efficiently catalyze selective DNA synthesis according to the Watson Crick rule in a templatedirected manner during DNA replication, repair, and recombination. Remarkably, some DNA polymerases achieve selective information transfer to the offspring in line with the Watson Crick rule with intrinsic error rates as low as one mistake per one million synthesized nucleotides.[1] This is far below the value that would be expected based on the energetic differences between canonical (i.e. Watson Crick) and noncanonical nucleobase pairing.[1] Geometric factors are widely cited to govern DNA polymerase selectivity.[1] Thus, high-fidelity DNA polymerases are believed to mostly select the canonical nucleotide based on close steric complementarity of the nascent base pair to the geometry of the active site of the enzyme.</dcterms:abstract>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/9636/1/Betz_Angew_Chem_Int_Ed_2010.pdf"/>
    <dc:creator>Exner, Thomas E.</dc:creator>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/9636"/>
    <dc:format>application/pdf</dc:format>
    <dcterms:title>Structures of DNA Polymerases caught processing size-augmented nucleotide probes</dcterms:title>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:contributor>Welte, Wolfram</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <dc:contributor>Streckenbach, Frank</dc:contributor>
    <dc:contributor>Exner, Thomas E.</dc:contributor>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dc:contributor>Marx, Andreas</dc:contributor>
    <dc:creator>Betz, Karin</dc:creator>
    <dc:contributor>Betz, Karin</dc:contributor>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/52"/>
    <dc:language>eng</dc:language>
    <dc:creator>Marx, Andreas</dc:creator>
    <dc:contributor>Schnur, Andreas</dc:contributor>
    <dc:creator>Diederichs, Kay</dc:creator>
    <dc:contributor>Diederichs, Kay</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T18:13:21Z</dc:date>
    <dc:creator>Streckenbach, Frank</dc:creator>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dc:creator>Schnur, Andreas</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <dc:creator>Welte, Wolfram</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/29"/>
    <dcterms:bibliographicCitation>First publ. in: Angewandte Chemie : International Edition ; 49 (2010), 30. - S. 5181-5184</dcterms:bibliographicCitation>
    <dcterms:issued>2010</dcterms:issued>
  </rdf:Description>
</rdf:RDF>
Interner Vermerk
xmlui.Submission.submit.DescribeStep.inputForms.label.kops_note_fromSubmitter
Kontakt
URL der Originalveröffentl.
Prüfdatum der URL
Prüfungsdatum der Dissertation
Finanzierungsart
Kommentar zur Publikation
Allianzlizenz
Corresponding Authors der Uni Konstanz vorhanden
Internationale Co-Autor:innen
Universitätsbibliographie
Ja
Begutachtet
Diese Publikation teilen