Zinc binding agonist effect on the recognition of the β-amyloid (4–10) epitope by anti-β-amyloid antibodies

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2004
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Zirah, Séverine
Stefanescu, Raluca
Tian, Xiaodan
Cecal, Roxana
Kozin, Sergey A.
Debey, Pascale
Rebuffat, Sylvie
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Biochemical and Biophysical Research Communications. 2004, 321(2), pp. 324-328. ISSN 0006-291X. Available under: doi: 10.1016/j.bbrc.2004.06.150
Zusammenfassung

Amyloid plaques associated to Alzheimer’s disease present a high content of zinc ions. We previously showed that the N-terminal region of the amyloid peptide Aβ constitutes an autonomous zinc-binding domain. This region encompasses the previously identified epitope Aβ(4–10) targeted by antibodies capable to reduce amyloid deposition, but the influence of Aβ/Zn binding on the epitope recognition remains unknown. We demonstrate here the effect of Zn2+ ions on the recognition of peptides sharing the sequence of the Aβ N-terminal domain, by two monoclonal antibodies recognizing the β-amyloid(4–10) epitope. The presence of Zn2+, but not of other cations, increased the recognition of the (1–16) peptide, while it was without effect on the recognition of the (1–10) peptide. These findings show a zinc-induced conformational change of the (1–16)-N-terminal region of Aβ, which results in a better accessibility of the Aβ(4–10) epitope to the anti-Aβ antibodies, and suggest a role of zinc in epitope-based vaccination approaches.

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540 Chemie
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Alzheimer’s disease, β-Amyloid peptide, Zinc binding, Epitope
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ISO 690ZIRAH, Séverine, Raluca STEFANESCU, Marilena MANEA, Xiaodan TIAN, Roxana CECAL, Sergey A. KOZIN, Pascale DEBEY, Sylvie REBUFFAT, Michael PRZYBYLSKI, 2004. Zinc binding agonist effect on the recognition of the β-amyloid (4–10) epitope by anti-β-amyloid antibodies. In: Biochemical and Biophysical Research Communications. 2004, 321(2), pp. 324-328. ISSN 0006-291X. Available under: doi: 10.1016/j.bbrc.2004.06.150
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@article{Zirah2004-08-20bindi-17609,
  year={2004},
  doi={10.1016/j.bbrc.2004.06.150},
  title={Zinc binding agonist effect on the recognition of the β-amyloid (4–10) epitope by anti-β-amyloid antibodies},
  number={2},
  volume={321},
  issn={0006-291X},
  journal={Biochemical and Biophysical Research Communications},
  pages={324--328},
  author={Zirah, Séverine and Stefanescu, Raluca and Manea, Marilena and Tian, Xiaodan and Cecal, Roxana and Kozin, Sergey A. and Debey, Pascale and Rebuffat, Sylvie and Przybylski, Michael}
}
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