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Synthesis and Antibody Recognition of Cyclic Epitope Peptides, Together with Their Dimer and Conjugated Derivatives Based on Residues 9-22 of Herpes Simplex Virus Type 1 Glycoprotein D

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2009

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Jakab, Annamária
Schlosser, Gitta
Feijlbrief, Matty
Welling-Wester, Sytske
Vila-Perello, Miquel
Andreu, David
Hudecz, Ferenc
Mezö, Gábor

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http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-94642
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https://doi.org/10.1021/bc800324g
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Bioconjugate Chemistry. 2009, 20(4), pp. 683-692. ISSN 1043-1802. eISSN 1520-4812. Available under: doi: 10.1021/bc800324g

Zusammenfassung

The synthesis of new cyclic peptides comprising the 9-22 epitope 9LKMADPNRFRGKDL22 sequence derived from HSV gD-1 is reported. In addition, we describe procedures for the preparation of cyclic peptide dimers and conjugates with an oligotuftsin derivative carrier. The binding of a monoclonal antibody, Mab A16, to the synthesized compounds was determined by enzyme-linked immunosorbent assay. It was demonstrated that cyclization decreased the binding activity of the antibody to the epitope. However, dimerization and conjugation could significantly increase the binding capacity of the cyclic epitope peptides. The attachment site in dimers and conjugates, as well as the topology of the construct, had a significant influence on the antibody recognition, while replacement of Met in position 11 by Nle had no marked effect.

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540 Chemie

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ISO 690JAKAB, Annamária, Gitta SCHLOSSER, Matty FEIJLBRIEF, Sytske WELLING-WESTER, Marilena MANEA, Miquel VILA-PERELLO, David ANDREU, Ferenc HUDECZ, Gábor MEZÖ, 2009. Synthesis and Antibody Recognition of Cyclic Epitope Peptides, Together with Their Dimer and Conjugated Derivatives Based on Residues 9-22 of Herpes Simplex Virus Type 1 Glycoprotein D. In: Bioconjugate Chemistry. 2009, 20(4), pp. 683-692. ISSN 1043-1802. eISSN 1520-4812. Available under: doi: 10.1021/bc800324g
BibTex
@article{Jakab2009Synth-3310,
  year={2009},
  doi={10.1021/bc800324g},
  title={Synthesis and Antibody Recognition of Cyclic Epitope Peptides, Together with Their Dimer and Conjugated Derivatives Based on Residues 9-22 of Herpes Simplex Virus Type 1 Glycoprotein D},
  number={4},
  volume={20},
  issn={1043-1802},
  journal={Bioconjugate Chemistry},
  pages={683--692},
  author={Jakab, Annamária and Schlosser, Gitta and Feijlbrief, Matty and Welling-Wester, Sytske and Manea, Marilena and Vila-Perello, Miquel and Andreu, David and Hudecz, Ferenc and Mezö, Gábor}
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