Intracellular Adenosine Triphosphate (ATP) Concentration : A Switch in the Decision Between Apoptosis and Necrosis

Leist, Marcel; Single, Barbara; Castoldi, Anna F.; Kühnle, Simone; Nicotera, Pierluigi

Intracellular Adenosine Triphosphate (ATP) Concentration : A Switch in the Decision Between Apoptosis and Necrosis

Dateien

jem_97.pdfGröße: 185.64 KBDownloads: 389

Datum

1997

Autor:innen

Leist, Marcel

Single, Barbara

Castoldi, Anna F.

Kühnle, Simone

Nicotera, Pierluigi

Open Access-Veröffentlichung

Open Access Green

Sammlungen

Biologie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

The Journal of Experimental Medicine. 1997, 185(8), pp. 1481-1486. ISSN 0022-1007. Available under: doi: 10.1084/jem.185.8.1481

Zusammenfassung

Apoptosis and necrosis are considered conceptually and morphologically distinct forms of cell death. Here, we report that demise of human T cells caused by two classic apoptotic triggers (staurosporin and CD95 stimulation) changed from apoptosis to necrosis, when cells were preemptied of adenosine triphosphate (ATP). Nuclear condensation and DNA fragmentation did not occur in cells predepleted of ATP and treated with either of the two inducers, although the kinetics of cell death were unchanged. Selective and graded repletion of the extramitochondrial ATP/pool with glucose prevented necrosis and restored the ability of the cells to undergo apoptosis. Pulsed ATP/depletion/repletion experiments also showed that ATP generation either by glycolysis or by mitochondria was required for the active execution of the final phase of apoptosis, which involves nuclear condensation and DNA degradation.

Fachgebiet (DDC)

570 Biowissenschaften, Biologie

Zitieren

ISO 690

LEIST, Marcel, Barbara SINGLE, Anna F. CASTOLDI, Simone KÜHNLE, Pierluigi NICOTERA, 1997. Intracellular Adenosine Triphosphate (ATP) Concentration : A Switch in the Decision Between Apoptosis and Necrosis. In: The Journal of Experimental Medicine. 1997, 185(8), pp. 1481-1486. ISSN 0022-1007. Available under: doi: 10.1084/jem.185.8.1481

BibTex

@article{Leist1997Intra-8688,
  year={1997},
  doi={10.1084/jem.185.8.1481},
  title={Intracellular Adenosine Triphosphate (ATP) Concentration : A Switch in the Decision Between Apoptosis and Necrosis},
  number={8},
  volume={185},
  issn={0022-1007},
  journal={The Journal of Experimental Medicine},
  pages={1481--1486},
  author={Leist, Marcel and Single, Barbara and Castoldi, Anna F. and Kühnle, Simone and Nicotera, Pierluigi}
}

RDF

<rdf:RDF
    xmlns:dcterms="http://purl.org/dc/terms/"
    xmlns:dc="http://purl.org/dc/elements/1.1/"
    xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#"
    xmlns:bibo="http://purl.org/ontology/bibo/"
    xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#"
    xmlns:foaf="http://xmlns.com/foaf/0.1/"
    xmlns:void="http://rdfs.org/ns/void#"
    xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > 
  <rdf:Description rdf:about="https://kops.uni-konstanz.de/server/rdf/resource/123456789/8688">
    <dc:creator>Leist, Marcel</dc:creator>
    <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8688/1/jem_97.pdf"/>
    <dc:creator>Kühnle, Simone</dc:creator>
    <foaf:homepage rdf:resource="http://localhost:8080/"/>
    <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/8688"/>
    <dc:language>eng</dc:language>
    <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <dc:creator>Single, Barbara</dc:creator>
    <dc:contributor>Castoldi, Anna F.</dc:contributor>
    <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:45:42Z</dc:date>
    <dc:format>application/pdf</dc:format>
    <dcterms:bibliographicCitation>First publ. in: The Journal of Experimental Medicine ; 185 (1997), 8. - pp. 1481-1486</dcterms:bibliographicCitation>
    <dc:contributor>Single, Barbara</dc:contributor>
    <dc:rights>terms-of-use</dc:rights>
    <dcterms:abstract xml:lang="eng">Apoptosis and necrosis are considered conceptually and morphologically distinct forms of cell death. Here, we report that demise of human T cells caused by two classic apoptotic triggers (staurosporin and CD95 stimulation) changed from apoptosis to necrosis, when cells were preemptied of adenosine triphosphate (ATP). Nuclear condensation and DNA fragmentation did not occur in cells predepleted of ATP and treated with either of the two inducers, although the kinetics of cell death were unchanged. Selective and graded repletion of the extramitochondrial ATP/pool with glucose prevented necrosis and restored the ability of the cells to undergo apoptosis. Pulsed ATP/depletion/repletion experiments also showed that ATP generation either by glycolysis or by mitochondria was required for the active execution of the final phase of apoptosis, which involves nuclear condensation and DNA degradation.</dcterms:abstract>
    <dc:creator>Castoldi, Anna F.</dc:creator>
    <dc:contributor>Nicotera, Pierluigi</dc:contributor>
    <dcterms:issued>1997</dcterms:issued>
    <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/8688/1/jem_97.pdf"/>
    <dc:contributor>Leist, Marcel</dc:contributor>
    <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:45:42Z</dcterms:available>
    <dc:contributor>Kühnle, Simone</dc:contributor>
    <dcterms:rights rdf:resource="https://rightsstatements.org/page/InC/1.0/"/>
    <dc:creator>Nicotera, Pierluigi</dc:creator>
    <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/server/rdf/resource/123456789/28"/>
    <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/>
    <dcterms:title>Intracellular Adenosine Triphosphate (ATP) Concentration : A Switch in the Decision Between Apoptosis and Necrosis</dcterms:title>
  </rdf:Description>
</rdf:RDF>

Universitätsbibliographie

Nein