Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians

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2005
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Vences, Miguel
Thomas, Meike
van der Meijden, Arie
Chiari, Ylenia
Vieites, David R.
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Frontiers in Zoology. 2005, 2(1), 5. ISSN 1742-9994. eISSN 1742-9994. Available under: doi: 10.1186/1742-9994-2-5
Zusammenfassung

Background

Identifying species of organisms by short sequences of DNA has been in the center of ongoing discussions under the terms DNA barcoding or DNA taxonomy. A C-terminal fragment of the mitochondrial gene for cytochrome oxidase subunit I (COI) has been proposed as universal marker for this purpose among animals.

Results

Herein we present experimental evidence that the mitochondrial 16S rRNA gene fulfills the requirements for a universal DNA barcoding marker in amphibians. In terms of universality of priming sites and identification of major vertebrate clades the studied 16S fragment is superior to COI. Amplification success was 100% for 16S in a subset of fresh and well-preserved samples of Madagascan frogs, while various combination of COI primers had lower success rates.COI priming sites showed high variability among amphibians both at the level of groups and closely related species, whereas 16S priming sites were highly conserved among vertebrates. Interspecific pairwise 16S divergences in a test group of Madagascan frogs were at a level suitable for assignment of larval stages to species (1–17%), with low degrees of pairwise haplotype divergence within populations (0–1%).

Conclusion

We strongly advocate the use of 16S rRNA as standard DNA barcoding marker for vertebrates to complement COI, especially if samples a priori could belong to various phylogenetically distant taxa and false negatives would constitute a major problem.

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ISO 690VENCES, Miguel, Meike THOMAS, Arie VAN DER MEIJDEN, Ylenia CHIARI, David R. VIEITES, 2005. Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians. In: Frontiers in Zoology. 2005, 2(1), 5. ISSN 1742-9994. eISSN 1742-9994. Available under: doi: 10.1186/1742-9994-2-5
BibTex
@article{Vences2005-03-16Compa-28164,
  year={2005},
  doi={10.1186/1742-9994-2-5},
  title={Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians},
  number={1},
  volume={2},
  issn={1742-9994},
  journal={Frontiers in Zoology},
  author={Vences, Miguel and Thomas, Meike and van der Meijden, Arie and Chiari, Ylenia and Vieites, David R.},
  note={Article Number: 5}
}
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