Interstitial dendritic cell guidance by haptotactic chemokine gradients

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2013
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Weber, Michele
Hauschild, Robert
Schwarz, Jan
Moussion, Christine
de Vries, Ingrid
Luther, Sanjiv A.
Bollenbach, Tobias
Sixt, Michael
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Science. 2013, 339(6117), pp. 328-332. ISSN 0036-8075. eISSN 1095-9203. Available under: doi: 10.1126/science.1228456
Zusammenfassung

Directional guidance of cells via gradients of chemokines is considered crucial for embryonic development, cancer dissemination, and immune responses. Nevertheless, the concept still lacks direct experimental confirmation in vivo. Here, we identify endogenous gradients of the chemokine CCL21 within mouse skin and show that they guide dendritic cells toward lymphatic vessels. Quantitative imaging reveals depots of CCL21 within lymphatic endothelial cells and steeply decaying gradients within the perilymphatic interstitium. These gradients match the migratory patterns of the dendritic cells, which directionally approach vessels from a distance of up to 90-micrometers. Interstitial CCL21 is immobilized to heparan sulfates, and its experimental delocalization or swamping the endogenous gradients abolishes directed migration. These findings functionally establish the concept of haptotaxis, directed migration along immobilized gradients, in tissues.

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ISO 690WEBER, Michele, Robert HAUSCHILD, Jan SCHWARZ, Christine MOUSSION, Ingrid DE VRIES, Daniel F. LEGLER, Sanjiv A. LUTHER, Tobias BOLLENBACH, Michael SIXT, 2013. Interstitial dendritic cell guidance by haptotactic chemokine gradients. In: Science. 2013, 339(6117), pp. 328-332. ISSN 0036-8075. eISSN 1095-9203. Available under: doi: 10.1126/science.1228456
BibTex
@article{Weber2013-01-18Inter-26341,
  year={2013},
  doi={10.1126/science.1228456},
  title={Interstitial dendritic cell guidance by haptotactic chemokine gradients},
  number={6117},
  volume={339},
  issn={0036-8075},
  journal={Science},
  pages={328--332},
  author={Weber, Michele and Hauschild, Robert and Schwarz, Jan and Moussion, Christine and de Vries, Ingrid and Legler, Daniel F. and Luther, Sanjiv A. and Bollenbach, Tobias and Sixt, Michael}
}
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