In situ EPR spectroscopy of a bacterial membrane transporter using an expanded genetic code

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Chemical Communications ; 57 (2021), 96. - S. 12980-12983. - Royal Society of Chemistry (RSC). - ISSN 1359-7345. - eISSN 1364-548X
Zusammenfassung
The membrane transporter BtuB is site-directedly spin labelled on the surface of living Escherichia coli via Diels–Alder click chemistry of the genetically encoded amino acid SCO-L-lysine. The previously introduced photoactivatable nitroxide PaNDA prevents off-target labelling, is used for distance measurements, and the temporally shifted activation of the nitroxide allows for advanced experimental setups. This study describes significant evolution of Diels–Alder-mediated spin labelling on cellular surfaces and opens up new vistas for the the study of membrane proteins.
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ISO 690KUGELE, Anandi, Sophie KETTER, Bjarne SILKENATH, Valentin WITTMANN, Benesh JOSEPH, Malte DRESCHER, 2021. In situ EPR spectroscopy of a bacterial membrane transporter using an expanded genetic code. In: Chemical Communications. Royal Society of Chemistry (RSC). 57(96), pp. 12980-12983. ISSN 1359-7345. eISSN 1364-548X. Available under: doi: 10.1039/d1cc04612h
BibTex
@article{Kugele2021-11-18spect-55826,
  year={2021},
  doi={10.1039/d1cc04612h},
  title={In situ EPR spectroscopy of a bacterial membrane transporter using an expanded genetic code},
  number={96},
  volume={57},
  issn={1359-7345},
  journal={Chemical Communications},
  pages={12980--12983},
  author={Kugele, Anandi and Ketter, Sophie and Silkenath, Bjarne and Wittmann, Valentin and Joseph, Benesh and Drescher, Malte}
}
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