In situ EPR spectroscopy of a bacterial membrane transporter using an expanded genetic code

Kugele, Anandi; Ketter, Sophie; Silkenath, Bjarne; Wittmann, Valentin; Joseph, Benesh; Drescher, Malte

In situ EPR spectroscopy of a bacterial membrane transporter using an expanded genetic code

Dateien

Kugele_2-40i8514f6nr3.pdfGröße: 2.13 MBDownloads: 99

Datum

2021

Autor:innen

Ketter, Sophie

Joseph, Benesh

Open Access-Veröffentlichung

Open Access Hybrid

Sammlungen

Chemie

Publikationstyp

Zeitschriftenartikel

Publikationsstatus

Published

Erschienen in

Chemical Communications. Royal Society of Chemistry (RSC). 2021, 57(96), pp. 12980-12983. ISSN 1359-7345. eISSN 1364-548X. Available under: doi: 10.1039/d1cc04612h

Zusammenfassung

The membrane transporter BtuB is site-directedly spin labelled on the surface of living Escherichia coli via Diels–Alder click chemistry of the genetically encoded amino acid SCO-L-lysine. The previously introduced photoactivatable nitroxide PaNDA prevents off-target labelling, is used for distance measurements, and the temporally shifted activation of the nitroxide allows for advanced experimental setups. This study describes significant evolution of Diels–Alder-mediated spin labelling on cellular surfaces and opens up new vistas for the the study of membrane proteins.

Fachgebiet (DDC)

540 Chemie

Zitieren

ISO 690

KUGELE, Anandi, Sophie KETTER, Bjarne SILKENATH, Valentin WITTMANN, Benesh JOSEPH, Malte DRESCHER, 2021. In situ EPR spectroscopy of a bacterial membrane transporter using an expanded genetic code. In: Chemical Communications. Royal Society of Chemistry (RSC). 2021, 57(96), pp. 12980-12983. ISSN 1359-7345. eISSN 1364-548X. Available under: doi: 10.1039/d1cc04612h

BibTex

@article{Kugele2021-11-18spect-55826,
  year={2021},
  doi={10.1039/d1cc04612h},
  title={In situ EPR spectroscopy of a bacterial membrane transporter using an expanded genetic code},
  number={96},
  volume={57},
  issn={1359-7345},
  journal={Chemical Communications},
  pages={12980--12983},
  author={Kugele, Anandi and Ketter, Sophie and Silkenath, Bjarne and Wittmann, Valentin and Joseph, Benesh and Drescher, Malte}
}

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