Petre, Brînduşa-Alina

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Petre
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Brînduşa-Alina
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Synthesis and characterization of a nonapeptides with specific properties for Cu binding

2007, Drochioiu, Gabi, Murariu, Manuela, Petre, Brînduşa-Alina, Manea, Marilena, Przybylski, Michael

A nonapeptide with the following sequence of amino acid residues CHQYHHNRE has been synthesized and characterized. The peptide is useful to investigate the binding of copper ions to histidine-containing peptides and proteins. It was synthesised manually by Solid Phase Peptide Synthesis (SPPS) using Fmoc protection strategy. The peptide was subjected to final purification by Reversed-Phase High Performance Liquid Chromatography (RP-HPLC) and characterised by matrix assisted laser desorption ionization with time of flight mass spectrometry (MALDI-TOF MS). The GPMAW 6.11 (General Protein/Mass Analysis for Window) program was used for theoretical characterization of the peptide.