Fischer, Jörg Wolfram Anselm
Site-directed attachment of photoexcitable spin labels for light-induced pulsed dipolar spectroscopy
2020-11-24, Williams, Lara, Tischlik, Sonja, Scherer, Andreas, Fischer, Jörg Wolfram Anselm, Drescher, Malte
Photoexcited triplet states are gaining popularity as spin labels in pulsed electron paramagnetic resonance (EPR) spectroscopy. Here, we demonstrate that the fluorophores Eosin Y, Rose Bengal and Atto Thio12 are suitable markers for distance determination by laser-induced magnetic dipole (LaserIMD) spectroscopy in proteins that lack an intrinsic photoexcitable center.
Gd(III)–Gd(III) Relaxation-Induced Dipolar Modulation Enhancement for In-Cell Electron Paramagnetic Resonance Distance Determination
2019-04-04, Azarkh, Mykhailo, Bieber, Anna, Qi, Mian, Fischer, Jörg Wolfram Anselm, Yulikov, Maxim, Godt, Adelheid, Drescher, Malte
In-cell distance determination by electron paramagnetic resonance (EPR) spectroscopy reveals essential structural information about biomacromolecules under native conditions. We demonstrate that the pulsed EPR technique RIDME (relaxation induced dipolar modulation enhancement) can be utilized for such distance determination. The performance of in-cell RIDME has been assessed at Q-band using stiff molecular rulers labeled with Gd(III)-PyMTA and microinjected into Xenopus laevis oocytes. The overtone coefficients are determined to be the same for protonated aqueous solutions and inside cells. As compared to in-cell DEER (double electron–electron resonance, also abbreviated as PELDOR), in-cell RIDME features approximately 5 times larger modulation depth and does not show artificial broadening in the distance distributions due to the effect of pseudosecular terms.
Morphogenesis of anisotropic nanoparticles : self-templating via non-classical, fibrillar Cd2Se intermediates
2018-06-28, Wurmbrand, Daniel, Fischer, Jörg Wolfram Anselm, Rosenberg, Rose, Boldt, Klaus
A fibrillar, polymeric intermediate (Cd2Se)n was isolated from the synthesis of CdSe nanorods, which suggests that the reactants themselves can template anisotropic growth. It is shown that high monomer concentration is the principal factor favouring this reaction pathway. The intermediate is distinct from crystalline semiconductor or small clusters and is surprisingly temperature-stable below 250 °C.