DNA Conjugation by Staudinger Ligation
| Type of Publication: | Journal article |
| URI (citable link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-76515 |
| Author: | Weisbrod, Samuel H.; Baccaro, Anna; Marx, Andreas |
| Year of publication: | 2008 |
| Published in: | Nucleic Acids Symposium Series ; 52 (2008), 1. - pp. 383-384 |
| Summary: |
Two 5 modified 2'-deoxyuridin triphosphates and a 7 modified 2'-deoxy-7-deazaadenosine were synthesized carrying a terminal azide linked to the base. For probing the sterical influence on incorporation and Staudinger ligation different sized flexible linkers were chosen. All three nucleotides can completely replace their natural counterparts in primer extension as well as polymerase chain reactions (PCR) using Pwo DNA polymerase. For azide labeled primer extension products subsequent conjugation of suitably functionalized phosphines via Staudinger ligation was achieved, e.g. for the conjugation of biotin as an affinity tag.
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| Subject (DDC): | 540 Chemistry |
| Link to License: | Terms of use |
| Bibliography of Konstanz: | Yes |
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WEISBROD, Samuel H., Anna BACCARO, Andreas MARX, 2008. DNA Conjugation by Staudinger Ligation. In: Nucleic Acids Symposium Series. 52(1), pp. 383-384
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