KOPS - The Institutional Repository of the University of Konstanz

N-Ethylmaleimide-Sensitive Factor Is Required to Organize Functional ExocytoticMicrodomains in Paramecium

N-Ethylmaleimide-Sensitive Factor Is Required to Organize Functional ExocytoticMicrodomains in Paramecium

Cite This

Files in this item

Checksum: MD5:9a426cfbcb1afef315f2de7b82fba76b

FROISSARD, Marine, Roland KISSMEHL, Jean-Claude DEDIEU, Tadeusz GULIK-KRZYWICKI, Helmut PLATTNER, Jean COHEN, 2002. N-Ethylmaleimide-Sensitive Factor Is Required to Organize Functional ExocytoticMicrodomains in Paramecium. In: Genetics. 161, pp. 643-650

@article{Froissard2002NEthy-7848, title={N-Ethylmaleimide-Sensitive Factor Is Required to Organize Functional ExocytoticMicrodomains in Paramecium}, year={2002}, volume={161}, journal={Genetics}, pages={643--650}, author={Froissard, Marine and Kissmehl, Roland and Dedieu, Jean-Claude and Gulik-Krzywicki, Tadeusz and Plattner, Helmut and Cohen, Jean} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/7848"> <dcterms:bibliographicCitation>First publ. in: Genetics ; 161 (2002). - S. 643-650</dcterms:bibliographicCitation> <dc:contributor>Plattner, Helmut</dc:contributor> <dc:creator>Plattner, Helmut</dc:creator> <dc:creator>Froissard, Marine</dc:creator> <dc:contributor>Cohen, Jean</dc:contributor> <dc:creator>Dedieu, Jean-Claude</dc:creator> <dcterms:title>N-Ethylmaleimide-Sensitive Factor Is Required to Organize Functional ExocytoticMicrodomains in Paramecium</dcterms:title> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dc:contributor>Gulik-Krzywicki, Tadeusz</dc:contributor> <dcterms:abstract xml:lang="eng">In exocytosis, secretory granules contact plasmamembrane at sites where microdomains can be observed, which are sometimes marked by intramembranous particle arrays. Such arrays are particularly obvious when membrane fusion is frozen at a subterminal stage, e.g., in neuromuscular junctions and ciliate exocytotic sites. In Paramecium, a genetic approach has shown thatthe rosettes of intramembranous particles are essential for stimulated exocytosis of secretory granules, the trichocysts. The identification of two genes encoding the N-ethylmaleimide-sensitive factor (NSF), a chaperone ATPase involved in organelle docking, prompted us to analyze its potential role in trichocyst exocytosis using a gene-silencing strategy. Here we show that NSF deprivation strongly interferes with rosette assembly but does not disturb the functioning of exocytotic sites already formed. We conclude that rosette organization involves ubiquitous partners of the fusion machinery and discuss where NSF could intervene in this mechanism.</dcterms:abstract> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7848/1/Factor_Is_Required_to_Organize_Functional.pdf"/> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:37:59Z</dc:date> <dc:format>application/pdf</dc:format> <dcterms:rights rdf:resource="https://creativecommons.org/licenses/by-nc-nd/2.0/legalcode"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2011-03-24T17:37:59Z</dcterms:available> <dc:creator>Cohen, Jean</dc:creator> <dc:creator>Kissmehl, Roland</dc:creator> <dc:contributor>Dedieu, Jean-Claude</dc:contributor> <dc:contributor>Froissard, Marine</dc:contributor> <dc:rights>terms-of-use</dc:rights> <dc:contributor>Kissmehl, Roland</dc:contributor> <dc:language>eng</dc:language> <dcterms:issued>2002</dcterms:issued> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <bibo:uri rdf:resource="http://kops.uni-konstanz.de/handle/123456789/7848"/> <dc:creator>Gulik-Krzywicki, Tadeusz</dc:creator> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/7848/1/Factor_Is_Required_to_Organize_Functional.pdf"/> </rdf:Description> </rdf:RDF>

Downloads since Oct 1, 2014 (Information about access statistics)

Factor_Is_Required_to_Organize_Functional.pdf 193

This item appears in the following Collection(s)

terms-of-use Except where otherwise noted, this item's license is described as terms-of-use

Search KOPS


Browse

My Account