Structurally different rat liver medium-chain acyl-CoA dehydrogenase directed by complementary DNA's differing in their 5'-region
| Publikationstyp: | Zeitschriftenartikel |
| URI (zitierfähiger Link): | http://nbn-resolving.de/urn:nbn:de:bsz:352-opus-53792 |
| Autor/innen: | Inagaki, Taisuke; Ohishi, Nobuko; Tsukagoshi, Norihiro; Udaka, Shigezo; Ghisla, Sandro; Yagi, Kunio |
| Erscheinungsjahr: | 1991 |
| Erschienen in: | Biochimica et Biophysica Acta / Protein Structure and Molecular Enzymology ; 1077 (1991), 3. - S. 285-290. - ISSN 0167-4838 |
| DOI (zitierfähiger Link): | https://dx.doi.org/10.1016/0167-4838(91)90542-8 |
| Zusammenfassung: |
Different forms of rat liver medium-chain acyl CoA dehydrogenase (MCAD) (EC 1.3.99.3) were produced in Escherichia coli carrying expression plasmids (pRMCADm-1−9) differing at the 5′-region of the cDNA. The proteins expressed could be readily extracted from the cells. The protein (not, vert, similar44 kDa) directed by pRMCADm-3 showed the highest activity and was readily purified to homogeneity. The purified enzyme contained non-covalently bound FAD and was similar to rat liver mitochondrial enzyme in all respects examined. The purified protein (not, vert, similar45 kDa) directed by pRMCADm-1 did not contain FAD and showed no enzymatic activity. Therefore, the leader peptide disturbs the binding of FAD to the apoprotein. The purified protein (not, vert, similar40 kDa) directed by pRMCADm-6 did not contain FAD. Thus, the deletion of the NH2-terminal portion of the apoprotein to some extent results in its inability to combine with FAD.
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| Fachgebiet (DDC): | 570 Biowissenschaften, Biologie |
| Schlagwörter: | Medium-chain, acyl CoA dehydrogenase, Complementary DNA, Rat liver |
| Link zur Lizenz: | Nutzungsbedingungen |
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INAGAKI, Taisuke, Nobuko OHISHI, Norihiro TSUKAGOSHI, Shigezo UDAKA, Sandro GHISLA, Kunio YAGI, 1991. Structurally different rat liver medium-chain acyl-CoA dehydrogenase directed by complementary DNA's differing in their 5'-region. In: Biochimica et Biophysica Acta / Protein Structure and Molecular Enzymology. 1077(3), pp. 285-290. ISSN 0167-4838. Available under: doi: 10.1016/0167-4838(91)90542-8
@article{Inagaki1991Struc-7735, title={Structurally different rat liver medium-chain acyl-CoA dehydrogenase directed by complementary DNA's differing in their 5'-region}, year={1991}, doi={10.1016/0167-4838(91)90542-8}, number={3}, volume={1077}, issn={0167-4838}, journal={Biochimica et Biophysica Acta / Protein Structure and Molecular Enzymology}, pages={285--290}, author={Inagaki, Taisuke and Ohishi, Nobuko and Tsukagoshi, Norihiro and Udaka, Shigezo and Ghisla, Sandro and Yagi, Kunio} }
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