KOPS - The Institutional Repository of the University of Konstanz

Evidence for an involvement of the ubiquitin-like modifier ISG15 in MHC class I antigen presentation

Evidence for an involvement of the ubiquitin-like modifier ISG15 in MHC class I antigen presentation

Cite This

Files in this item

Files Size Format View

There are no files associated with this item.

HELD, Tobias, Michael BASLER, Klaus-Peter KNOBELOCH, Marcus GROETTRUP, 2020. Evidence for an involvement of the ubiquitin-like modifier ISG15 in MHC class I antigen presentation. In: European Journal of Immunology. Wiley. ISSN 0014-2980. eISSN 1521-4141. Available under: doi: 10.1002/eji.202048646

@article{Held2020-07-20Evide-50595, title={Evidence for an involvement of the ubiquitin-like modifier ISG15 in MHC class I antigen presentation}, year={2020}, doi={10.1002/eji.202048646}, issn={0014-2980}, journal={European Journal of Immunology}, author={Held, Tobias and Basler, Michael and Knobeloch, Klaus-Peter and Groettrup, Marcus} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/50595"> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Basler, Michael</dc:creator> <dc:creator>Held, Tobias</dc:creator> <dc:contributor>Held, Tobias</dc:contributor> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dc:creator>Groettrup, Marcus</dc:creator> <dcterms:title>Evidence for an involvement of the ubiquitin-like modifier ISG15 in MHC class I antigen presentation</dcterms:title> <dc:contributor>Basler, Michael</dc:contributor> <dc:contributor>Knobeloch, Klaus-Peter</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-08-27T11:56:05Z</dc:date> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:issued>2020-07-20</dcterms:issued> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2020-08-27T11:56:05Z</dcterms:available> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/50595"/> <dc:contributor>Groettrup, Marcus</dc:contributor> <dc:creator>Knobeloch, Klaus-Peter</dc:creator> <dcterms:abstract xml:lang="eng">The IFN stimulated gene 15 (ISG15) encodes a 15-kDa ubiquitin-like protein, that is induced by type I IFNs and is conjugated to the bulk of newly synthesized polypeptides at the ribosome. ISG15 functions as an antiviral molecule possibly by being covalently conjugated to viral proteins and disturbing virus particle assembly. Here, we have investigated the effect of ISGylation on degradation and antigen presentation of viral and cellular proteins. ISGylation did not induce proteasomal degradation of bulk ISG15 target proteins neither after overexpressing ISG15 nor after induction by IFN-β. The MHC class I cell surface expression of splenocytes derived from ISG15-deficient mice or mice lacking the catalytic activity of the major de-ISGylating enzyme USP18 was unaltered as compared to WT mice. Fusion of ubiquitin or FAT10 to the long-lived nucleoprotein (NP) of lymphocytic choriomeningitis virus accelerated the proteasomal degradation of NP while fusion to ISG15 did not detectably speed up NP degradation. Nevertheless, MHC-I restricted presentation of two epitopes of NP were markedly enhanced when it was fused to ISG15 similarly to fusion with ubiquitin or FAT10. Thus, we provide evidence that ISG15 can enhance the presentation of antigens on MHC-I most likely by promoting co-translational antigen processing.</dcterms:abstract> <dc:language>eng</dc:language> </rdf:Description> </rdf:RDF>

This item appears in the following Collection(s)

Search KOPS


Browse

My Account