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HIV taken by STORM : Super-resolution fluorescence microscopy of a viral infection

HIV taken by STORM : Super-resolution fluorescence microscopy of a viral infection

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Prüfsumme: MD5:7629f9539383bed4206ce074c5ee38da

PEREIRA, Cândida F., Jérémie ROSSY, Dylan M. OWEN, Johnson MAK, Katharina GAUS, 2012. HIV taken by STORM : Super-resolution fluorescence microscopy of a viral infection. In: Virology journal. 9, 84. eISSN 1743-422X. Available under: doi: 10.1186/1743-422X-9-84

@article{Pereira2012taken-43472, title={HIV taken by STORM : Super-resolution fluorescence microscopy of a viral infection}, year={2012}, doi={10.1186/1743-422X-9-84}, volume={9}, journal={Virology journal}, author={Pereira, Cândida F. and Rossy, Jérémie and Owen, Dylan M. and Mak, Johnson and Gaus, Katharina}, note={Article Number: 84} }

Background<br />The visualization of viral proteins has been hindered by the resolution limit of conventional fluorescent microscopes, as the dimension of any single fluorescent signal is often greater than most virion particles. Super-resolution microscopy has the potential to unveil the distribution of proteins at the resolution approaching electron microscopy without relying on morphological features of existing characteristics of the biological specimen that are needed in EM.<br /><br />Results<br />Using direct stochastic optical reconstruction microscopy (dSTORM) to achieve a lateral resolution of 15–20 nm, we quantified the 2-D molecular distribution of the major structural proteins of the infectious human immunodeficiency virus type 1 (HIV-1) before and after infection of lymphoid cells. We determined that the HIV-1 matrix and capsid proteins undergo restructuring soon after HIV-1 infection.<br />Conclusions<br />This study provides the proof-of-concept for the use of dSTORM to visualize the changes in the molecular distribution of viral proteins during an infection. 2012 Owen, Dylan M. 2018-10-10T08:24:13Z Mak, Johnson 2018-10-10T08:24:13Z Pereira, Cândida F. eng Rossy, Jérémie Owen, Dylan M. Gaus, Katharina HIV taken by STORM : Super-resolution fluorescence microscopy of a viral infection Gaus, Katharina terms-of-use Pereira, Cândida F. Mak, Johnson Rossy, Jérémie

Dateiabrufe seit 10.10.2018 (Informationen über die Zugriffsstatistik)

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