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Sequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigen

Sequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigen

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SCHEFFNER, Martin, Rainer WESSEL, Hans STAHL, 1989. Sequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigen. In: Nucleic Acids Research. 17(1), pp. 93-106. ISSN 0301-5610. eISSN 1362-4962. Available under: doi: 10.1093/nar/17.1.93

@article{Scheffner1989Seque-42843, title={Sequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigen}, year={1989}, doi={10.1093/nar/17.1.93}, number={1}, volume={17}, issn={0301-5610}, journal={Nucleic Acids Research}, pages={93--106}, author={Scheffner, Martin and Wessel, Rainer and Stahl, Hans} }

<rdf:RDF xmlns:dcterms="http://purl.org/dc/terms/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:rdf="http://www.w3.org/1999/02/22-rdf-syntax-ns#" xmlns:bibo="http://purl.org/ontology/bibo/" xmlns:dspace="http://digital-repositories.org/ontologies/dspace/0.1.0#" xmlns:foaf="http://xmlns.com/foaf/0.1/" xmlns:void="http://rdfs.org/ns/void#" xmlns:xsd="http://www.w3.org/2001/XMLSchema#" > <rdf:Description rdf:about="https://kops.uni-konstanz.de/rdf/resource/123456789/42843"> <dcterms:available rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-07-11T09:59:06Z</dcterms:available> <dcterms:title>Sequence independent duplex DNA opening reaction catalysed by SV40 large tumor antigen</dcterms:title> <dcterms:issued>1989</dcterms:issued> <void:sparqlEndpoint rdf:resource="http://localhost/fuseki/dspace/sparql"/> <dcterms:isPartOf rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dspace:hasBitstream rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/42843/3/Scheffner_2-f8cp9lug6vhd7.pdf"/> <bibo:uri rdf:resource="https://kops.uni-konstanz.de/handle/123456789/42843"/> <dc:contributor>Scheffner, Martin</dc:contributor> <dc:creator>Stahl, Hans</dc:creator> <dc:contributor>Stahl, Hans</dc:contributor> <dc:date rdf:datatype="http://www.w3.org/2001/XMLSchema#dateTime">2018-07-11T09:59:06Z</dc:date> <dc:rights>terms-of-use</dc:rights> <dc:contributor>Wessel, Rainer</dc:contributor> <dcterms:abstract xml:lang="eng">Simian virus 40 (SV40) large tumor antigen (T antigen) is mainly localized in the nucleus where it exhibits two biochemical properties: DNA binding and helicase activity. Both activities are necessary for viral DNA replication and may also enable T antigen to modulate cellular growth. Here we present biochemical and electron microscopic evidence that the helicase activity can start at internal sites of fully double-stranded DNA molecules not containing the SV40 origin or replication. Using T antigen specific monoclonal antibodies, this unwinding reaction can be biochemically divided in an initiation (duplex opening) and a propagation step. The duplex opening reaction (as well as the propagation step) does not depend on a specific DNA sequence or secondary structure. In addition, we have found that T antigen forms an ATP dependent nucleoprotein complex at double-stranded DNA, which may be an essential step for the sequence independent duplex DNA opening reaction.</dcterms:abstract> <dc:creator>Scheffner, Martin</dc:creator> <dspace:isPartOfCollection rdf:resource="https://kops.uni-konstanz.de/rdf/resource/123456789/28"/> <dcterms:hasPart rdf:resource="https://kops.uni-konstanz.de/bitstream/123456789/42843/3/Scheffner_2-f8cp9lug6vhd7.pdf"/> <dcterms:rights rdf:resource="https://kops.uni-konstanz.de/page/termsofuse"/> <dc:language>eng</dc:language> <foaf:homepage rdf:resource="http://localhost:8080/jspui"/> <dc:creator>Wessel, Rainer</dc:creator> </rdf:Description> </rdf:RDF>

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